LOCUS       sequence-285735-        1463 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3064
FEATURES             Location/Qualifiers
     source          1..1463
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1295..1319)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1327..1368
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggaggatgt ttcctcagcg
       61 gccccgagcc cgcggggctg tgcggatggt agggatgccg accctactga ggagcagatg
      121 gcagaaacag agagaaacga cgaggagcag ttcgaatgcc aggaactgct cgagtgccag
      181 gtgcaggtgg gggcccccga ggaggaggag gaggaggagg aggacgcggg cctggtggcc
      241 gaggccgagg ccgtggctgc cggctggatg ctcgatttcc tctgcctctc tctttgccga
      301 gctttccgcg acggccgctc cgaggacttc cgcaggaccc gcaacagcgc agaggctatt
      361 attcatggac tatccagtct aacagcttgc cagttgagaa cgatatacat atgtcagttt
      421 ttgacaagaa ttgcagcagg aaaaaccctt gatgcacagt ttgaaaatga tgaacgaatt
      481 acacccttgg aatcagccct gatgatttgg ggttcaattg aaaaggaaca tgacaaactt
      541 catgaagaaa tacagaattt aattaaaatt caggctatag ctgtttgtat ggaaaatggc
      601 aactttaaag aagcagaaga agtctttgaa agaatatttg gtgatccaaa ttctcatatg
      661 cctttcaaaa gcaaattgct tatgataatc tctcagaaag atacatttca ttcctttttt
      721 caacacttca gctacaacca catgatggag aaaattaaga gttatgtgaa ttatgtgcta
      781 agtgaaaaat catcaacctt tctaatgaag gcagcggcaa aagtagtaga aagcaaaagg
      841 acaagaacaa taacttctca agataaacct agtggtaatg atgttgaaat ggaaactgaa
      901 gctaatttgg atacaagaaa aaggtctcac aagaatcttt tcttatctaa gttgcaacat
      961 ggaacccagc aacaagacct taataagaaa gaaagaagag taggaactcc tcaaagtaca
     1021 aaaaagaaaa aagaaagcag aagagccact gaaagcagaa tacctgtttc aaagagtcag
     1081 ccggtaactc ctgaaaaaca tcgagctaga aaaagacagg catggctttg ggaagaagac
     1141 aagaatttga gatctggcgt gaggaaatat ggagagggaa actggtctaa aatactgttg
     1201 cattataaat tcaacaaccg gacaagtgtc atgttaaaag acagatggag gaccatgaag
     1261 aaactaaaac tgatttcctc agacagcgaa gactgcccaa ctttcttgta caaagtggtt
     1321 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1381 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     1441 ttcaagtctc cctctataaa tat
//
