LOCUS       sequence-285738-        2345 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3065
FEATURES             Location/Qualifiers
     source          1..2345
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2177..2201)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2209..2250
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg caaagcccag ccacagcagc
       61 tatgtccttc agcagctaaa caaccaaaga gaatggggtt ttctctgtga ctgctgtatt
      121 gcaattgatg acatttactt tcaagcacac aaggcagttc tagctgcctg tagctcctat
      181 tttagaatgt ttttcatgaa ccatcagcat agtactgcac aactgaatct cagcaacatg
      241 aaaattagtg cagaatgttt tgatctcatt ttgcagttta tgtatttagg aaaaattatg
      301 acagctccct ccagttttga gcagtttaaa gtggcaatga actacctaca gctatacaat
      361 gttcctgact gtttagaaga catccaggat gcagattgtt ctagttcaaa atgttcctct
      421 tctgcttcca gcaaacagaa cagcaaaatg atatttgggg taagaatgta tgaagatact
      481 gtggctcgaa atggcaatga agccaacagg tggtgtgcag agccaagttc aacggtaaat
      541 acaccacata atagagaggc tgatgaagag tctttacaat taggtaattt tcctgagcca
      601 ctatttgatg tatgtaaaaa aagttccgtg tccaaattat ctactccaaa agaacgtgtg
      661 tcaagacgct ttgggcggag ttttacctgt gatagctgtg gatttggctt tagctgtgaa
      721 aaattattag atgagcatgt gctaacctgt actaacagac atttatacca aaacacaaga
      781 tcttaccata gaatagtaga tattagagat ggaaaagaca gtaacatcaa agctgaattt
      841 ggtgaaaaag attcttccaa aacattttct gcacagacgg acaaatacag aggagacaca
      901 agccaggctg ctgatgattc agcttcaacc actggaagca gaaaaagtag cacagtggag
      961 tctgaaatag caagcgaaga gaaaagcaga gctgctgaga ggaaaaggat tattattaag
     1021 atggagccag aagatattcc tacagatgaa ctgaaagact ttaacattat taaagttact
     1081 gataaagact gtaatgaatc cactgacaat gatgaattag aagatgaacc tgaagagcca
     1141 ttttatagat actatgttga agaagatgtc agcataaaaa aaagtggtag gaaaactcta
     1201 aaacctcgaa tgtcagtaag tgctgatgaa agaggtggtt tagagaatat gaggccccct
     1261 aacaacagca gtccagtaca agaggatgct gaaaatgcat cttgtgagct gtgtggactt
     1321 acaataaccg aggaggacct gtcatctcat tacttagcca aacacattga aaatatctgt
     1381 gcatgtggta aatgtggaca aatacttgta aagggtaggc agcttcagga acatgctcaa
     1441 cgatgtggcg agccccaaga tctgaccatg aatgggttag gaaatactga ggagaaaatg
     1501 gacttggaag agaatcctga tgagcagtcc gaaataagag atatgtttgt tgaaatgctg
     1561 gatgatttta gggacaatca ttaccagata aacagtatcc aaaaaaagca gttatttaaa
     1621 cattctgcct gcccttttcg atgtcctaat tgtggccagc gttttgaaac tgaaaatcta
     1681 gtggttgaac atatgtctag ctgcttagat caagatatgt ttaagagtgc catcatggaa
     1741 gaaaatgaaa gagatcacag acgaaagcat ttttgtaatc tgtgtggaaa aggattttat
     1801 cagcggtgtc acttaagaga acactatact gttcatacta aggaaaaaca gtttgtttgt
     1861 caaacatgtg gaaagcagtt tttaagagag cgtcagttgc gactgcacaa tgatatgcac
     1921 aaaggcatgg ccaggtatgt ctgttccatt tgtgatcaag gaaacttcag aaaacatgac
     1981 catgtacggc atatgatttc tcatttatct gctggtgaga ctatatgcca ggtctgcttt
     2041 cagatattcc caaataatga acagttggag cagcacatgg atgttcatct gtatacatgt
     2101 ggaatatgtg gagcaaaatt taatttgagg aaagatatga gatcacatta taatgccaag
     2161 catttgaaaa gaacctgccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     2221 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     2281 atttcgattt cttggcttta tatatcttgt ggaaaggacg acggtaatgc gtgccaatca
     2341 atgcg
//
