LOCUS       sequence-285741-        2186 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3066
FEATURES             Location/Qualifiers
     source          1..2186
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2018..2042)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2050..2091
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agggagatgg ttcagaccca
       61 gagcctccag atgccgggga ggacagcaag tccgagaatg gggagaatgc gcccatctac
      121 tgcatctgcc gcaaaccgga catcaactgc ttcatgatcg ggtgtgacaa ctgcaatgag
      181 tggttccatg gggactgcat ccggatcact gagaagatgg ccaaggccat ccgggagtgg
      241 tactgtcggg agtgcagaga gaaagacccc aagctagaga ttcgctatcg gcacaagaaa
      301 tcacgggagc gggatggcaa tgagcgggac agcagtgagc cccgggatga gggtggaggg
      361 cgcaagaggc ctgtccctga tccagacctg cagcgccggg cagggtcagg gacaggggtt
      421 ggggccatgc ttgctcgggg ctctgcttcg ccccacaaat cctctccgca gcccttggtg
      481 gccacaccca gccagcatca ccagcagcag cagcagcaga tcaaacggtc agcccgcatg
      541 tgtggtgagt gtgaggcatg tcggcgcact gaggactgtg gtcactgtga tttctgtcgg
      601 gacatgaaga agttcggggg ccccaacaag atccggcaga agtgccggct gcgccagtgc
      661 cagctgcggg cccgggaatc gtacaagtac ttcccttcct cgctctcacc agtgacgccc
      721 tcagagtccc tgccaaggcc ccgccggcca ctgcccaccc aacagcagcc acagccatca
      781 cagaagttag ggcgcatccg tgaagatgag ggggcagtgg cgtcatcaac agtcaaggag
      841 cctcctgagg ctacagccac acctgagcca ctctcagatg aggacctacc tctggatcct
      901 gacctgtatc aggacttctg tgcaggggcc tttgatgacc atggcctgcc ctggatgagc
      961 gacacagaag agtccccatt cctggacccc gcgctgcgga agagggcagt gaaagtgaag
     1021 catgtgaagc gtcgggagaa gaagtctgag aagaaggtga tggagaggaa ggaggagcga
     1081 tacaagcggc atcggcagaa gcagaagcac aaggataaat ggaaacaccc agagagggct
     1141 gatgccaagg accctgcgtc actgccccag tgcctggggc ccggctgtgt gcgccccgcc
     1201 cagcccagct ccaagtattg ctcagatgac tgtggcatga agctggcagc caaccgcatc
     1261 tacgagatcc tcccccagcg catccagcag tggcagcaga gcccttgcat tgctgaagag
     1321 cacggcaaga agctgctcga acgcattcgc cgagagcagc agagtgcccg cacccgcctt
     1381 caggaaatgg aacgccgatt ccatgagctt gaggccatca ttctacgtgc caagcagcag
     1441 gctgtgcgcg aggatgagga gagcaacgag ggtgacagtg atgacacaga cctgcagatc
     1501 ttctgtgttt cctgtgggca ccccatcaac ccacgtgttg ccttgcgcca catggagcgc
     1561 tgctacgcca agtatgagag ccagacgtcc tttgggtcca tgtaccccac acgcattgaa
     1621 ggggccacac gactcttctg tgatgtgtat aatcctcaga gcaaaacata ctgtaagcgg
     1681 ctccaggtgc tgtgccccga gcactcacgg gaccccaaag tgccagctga cgaggtatgc
     1741 gggtgccccc ttgtacgtga tgtctttgag ctcacgggtg acttctgccg cctgcccaag
     1801 cgccagtgca atcgccatta ctgctgggag aagctgcggc gtgcggaagt ggacttggag
     1861 cgcgtgcgtg tgtggtacaa gctggacgag ctgtttgagc aggagcgcaa tgtgcgcaca
     1921 gccatgacaa accgcgcggg attgctggcc ctgatgctgc accagacgat ccagcacgat
     1981 cccctcacta ccgacctgcg ctccagtgcc gaccgctgcc caactttctt gtacaaagtg
     2041 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     2101 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     2161 gaacattaca acagctgaca acactc
//
