LOCUS       sequence-285747-        1931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3068
FEATURES             Location/Qualifiers
     source          1..1931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(727..738)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1763..1787)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1795..1836
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcttctga cctagagagt
       61 agcctcacct ccatagactg gctcccccag ctgaccctcc gagctaccat tgagaagctt
      121 ggaagtgcct cccaggctgg gcctcccggg agcagccgca agtgttcacc agggtcaccc
      181 acagatccta atgccaccct gagcaaagac gaggcagcag tgcaccagga cggcaagcca
      241 cgatacagct atgccactct catcacctat gccatcaact cctctccagc caagaagatg
      301 accctcagcg agatttaccg ctggatctgt gataacttcc cctattacaa gaatgctggc
      361 attggttgga agaattcaat acggcacaac ctttctctca acaagtgttt ccggaaggtg
      421 cccagacctc gggatgaccc tgggaagggt tcctattgga caattgacac ctgccctgac
      481 atttcccgaa agagaagaca ccctccagat gatgatctgt cccaagactc accagaacag
      541 gaggcaagca agagcccacg gggaggcgtt gcagggagtg gagaagcctc actgcctcct
      601 gaggggaatc cgcagatgtc acttcagagc cccacatcta tagccagcta cagccagggc
      661 acaggatctg tggatggtgg agcagtggca gcaggggctt caggccgaga aagtgctgag
      721 ggtccccctc ccctctataa caccaaccat gactttaaat tctcctactc agagatcaac
      781 tttcaggatc taagctggtc cttccgcaac ctctataagt ccatgctgga gaagtcctct
      841 tcctcctctc agcacggctt ttcttctctc ctgggggaca tcccaccctc gaacaactac
      901 tacatgtatc agcagcagca gccaccgcca cctcaacagc agcagcagca gcagcagccg
      961 ccacagccac ctccccagca gtcccagcca cagcagcagc aggcacctgc ccagggcccc
     1021 tcagctgtag ggggtgctcc tccactgcac accccaagca cagatggttg taccccacca
     1081 gggggaaagc aagctggggc ggaaggctat gggcctcccc ctgtaatggc catgcatcca
     1141 cccccgctgc agcatggagg ctaccaccct catcagcacc atccccactc ccaccctgcc
     1201 cagcagccac cacctccaca gccacaggca caaggccagg ctcccatcaa caacactggc
     1261 tttgcctttc cttctgactg gtgctctaat attgactctt taaaggaaag cttcaagatg
     1321 gtgaatcggc tcaattggtc cagcattgag cagtcacaat tctcagaact gatggagagt
     1381 ctacgacagg cagagcagaa gaactggacc ctcgaccagc atcacattgc caatctgtgt
     1441 gactccctca accacttcct tactcagact ggtcacgtgc cccctcaagg gggtacccac
     1501 cgcccaccag cccctgcccg tattgctgac tcctgtgccc tcaccagtgg caaacaggag
     1561 tcagccatga gccaagtgaa ctcttatggg cacccacaag ctccccacct ctaccctggc
     1621 ccatcaccaa tgtacccaat ccccacccag gactcagcag gatacaatcg cccagcacac
     1681 catatggtcc ctcggccatc agtgccacct cctggtgcca atgaggagat ccctgatgac
     1741 ttcgactggg acttgatcac tttgccaact ttcttgtaca aagtggttga tatcggtaag
     1801 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1861 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaggt agcaagtgca
     1921 gtcagctccg g
//
