LOCUS       sequence-285750-        1379 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3069
FEATURES             Location/Qualifiers
     source          1..1379
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1211..1235)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1243..1284
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggctgtggt ggagaatgta
       61 gtgaagctcc ttggggagca gtactacaaa gatgccatgg agcagtgcca caattacaat
      121 gctcgcctct gtgctgagcg cagcgtgcgc ctgcctttct tggactcaca gaccggagta
      181 gcccagagca attgttacat ctggatggaa aagcgacacc ggggtccagg attggcctcc
      241 ggacagctgt actcctaccc tgcccggcgc tggcggaaaa agcggcgagc ccatccccct
      301 gaggatccac gactttcctt cccatctatt aagccagaca cagaccagac cctgaagaag
      361 gaggggctga tctctcagga tggcagtagt ttagaggctc tgttgcgcac tgaccccctg
      421 gagaagcgag gtgccccgga tccccgagtt gatgatgaca gcctgggcga gtttcctgtg
      481 accaacagtc gagcgcgaaa gcggatccta gaaccagatg acttcctgga tgacctcgat
      541 gatgaagact atgaagaaga tactcccaag cgtcggggaa aggggaaatc caagggtaag
      601 ggtgtgggca gtgcccgtaa gaagctggat gcttccatcc tggaggaccg ggataagccc
      661 tatgcctgtg acatttgtgg aaaacgttac aagaaccgac caggcctcag ttaccactat
      721 gcccactccc acttggctga ggaggagggc gaggacaagg aagactctca accacccact
      781 cctgtttccc agaggtctga ggagcagaaa tccaaaaagg gtcctgatgg attggccttg
      841 cccaacaact actgtgactt ctgcctgggg gactcaaaga ttaacaagaa gacgggacaa
      901 cccgaggagc tggtgtcctg ttctgactgt ggccgctcag ggcatccatc ttgcctccaa
      961 tttacccccg tgatgatggc ggcagtgaag acataccgct ggcagtgcat cgagtgcaaa
     1021 tgttgcaata tctgcggcac ctccgagaat gacgaccagt tgctcttctg tgatgactgc
     1081 gatcgtggct accacatgta ctgtctcacc ccgtccatgt ctgagccccc tgaaggaagt
     1141 tggagctgcc acctgtgtct ggacctgttg aaagagaaag cttccatcta ccagaaccag
     1201 aactcctctt gcccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1261 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1321 atttcttggc tttatatatc ttgtggaaag gacgatacta gcaccacaaa aacaggagc
//
