LOCUS       sequence-285753-        1412 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3070
FEATURES             Location/Qualifiers
     source          1..1412
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1244..1268)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1276..1317
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt acacttcaga agagaaatgt
       61 aatcagagaa ctcaaaaaag gaaaatatat aatgtatgcc ctcggaaggg taaaaagatt
      121 tttattcata tgcatgagat tattcagata gatggtcata tataccagtg ccttgaatgc
      181 aagcaaaact tctgtgaaaa cttagctctt attatgtgtg agagaaccca tactggggag
      241 aaaccttata aatgtgatat gtgtgagaaa acctttgtcc aaagctcaga tcttacttca
      301 caccagagga tccacaatta cgagaaacct tataaatgta gcaaatgtga gaagagcttt
      361 tggcatcact tagcgctttc aggacatcag agaacacatg caggtaaaaa attctataca
      421 tgtgacattt gtggcaagaa ttttggtcag agttctgatc tgcttgtcca ccagcgaagc
      481 catactggcg agaaaccata tctatgtagt gagtgtgaca aatgcttcag tagaagtaca
      541 aacctcataa ggcatcgaag aactcacaca ggtgagaaac catttaagtg tctcgagtgt
      601 gaaaaagctt ttagtgggaa atcagatctt attagccacc agagaactca cactggggaa
      661 aggccctaca aatgtaataa gtgtgagaaa agttaccgac accgttcagc cttcattgta
      721 cataaaagag ttcatactgg ggagaagccc tataagtgtg gtgcctgtga aaaatgcttt
      781 ggccagaaat cagaccttat cgtgcaccag agagtccaca caggtgagaa gccgtataaa
      841 tgcctggaat gtatgagaag ttttactcgg agtgccaacc taattaggca ccaggcaact
      901 cacactcaca cttttaaatg ccttgaatat gaaaaaagct ttaactgtag ctcagatctt
      961 attgtacatc agagaattca catggaagag aaaccacatc agtggtctgc gtgtgagagt
     1021 ggcttcctcc taggaatgga ctttgttgcc caacagaaaa tgagaactca aacagaggag
     1081 ctacactata aatacactgt atgtgataaa agcttccacc agagttcagc ccttcttcaa
     1141 catcagacag tacacattgg tgaaaaaccg tttgtctgta atgtgagtga aaaaggtctt
     1201 gagcttagcc ctccccatgc gtcagaagcc tcacagatgt cttgcccaac tttcttgtac
     1261 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1321 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1381 aaggacgatc acaactcaac ttatgccctg gt
//
