LOCUS       sequence-285756-        2006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3071
FEATURES             Location/Qualifiers
     source          1..2006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1838..1862)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1870..1911
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgctgggcat gtacgtgccg
       61 gacaggttct ccctgaagtc ctcccgggtt caggacggca tggggctcta cacggcccgc
      121 agagtgcgaa agggtgaaaa gttcggaccc tttgctggag agaagagaat gcctgaagac
      181 ttggatgaaa atatggatta caggttgatg tgggaggttc gtgggagtaa gggagaagtt
      241 ttgtacattt tggatgctac caacccacgg cactccaact ggcttcgctt cgttcatgag
      301 gcaccatctc aggagcagaa gaacttggct gccattcaag aaggagaaaa cattttctat
      361 ttggcagttg aagatataga aacagacacg gagcttctga ttggctacct ggatagtgac
      421 atggaggctg aggaggaaga acagcaaatt atgacagtca tcaaagaagg ggaagttgaa
      481 aattctagaa gacaatcaac agcgggcaga aaagatcgcc ttggctgtaa agaggactat
      541 gcttgtcctc aatgtgaatc gagttttacc agtgaggata ttcttgctga gcatctccag
      601 acattgcacc agaaacccac agaggagaaa gaatttaagt gcaagaactg tgggaagaaa
      661 ttcccagtta agcaggcttt gcaaagacat tttgagcagc accaggagac ttgccggggg
      721 gatgccaggt ttgtgtgcaa ggctgacagc tgtggaaaga ggctgaagag caaggatgcc
      781 ctgaaaagac accaggaaaa tgtccacact ggagatccta agaaaaagct tatatgttca
      841 gtgtgcaata aaaagtgttc ttcagcatca agcctacagg aacatagaaa gattcatgag
      901 atatttgatt gtcaagaatg tatgaagaaa tttatttcag ctaatcagct aaaacgtcat
      961 atgatcaccc actcagaaaa acgaccctat aattgcgaga tttgtaataa gtctttcaag
     1021 aggcttgatc aagtgggtgc tcacaaagta atacacagcg aagacaaacc ttacaaatgc
     1081 aaactttgtg gaaagggatt tgcccacaga aatgtttaca agaatcataa gaagacccac
     1141 tctgaggaga gaccgttcca atgtgaagaa tgtaaagctc tgttccggac cccattttct
     1201 ttacagagac acctgctaat acataacagt gagaggactt tcaagtgcca tcactgcgat
     1261 gctaccttta agaggaagga tacattaaat gttcatgtcc aggtggttca tgaaagacac
     1321 aagaagtata ggtgtgagct atgtaataag gcctttgtta caccttcagt gcttagaagt
     1381 cataagaaaa cacatacagg agaaaaggag aaaatctgtc catattgtgg ccagaaattt
     1441 gccagcagtg gtacactcag agttcatatc cggagccaca caggtgagcg tccctatcaa
     1501 tgtccttact gtgaaaaagg attcagtaaa aatgatggac tgaagatgca cattcgtact
     1561 cacaccaggg agaagccgta caagtgctca gagtgcagca aggccttcag ccagaagcga
     1621 ggcctggatg agcacaagag gacgcacact ggagaaaagc cttttcagtg tgatgtttgt
     1681 gatttggctt ttagcctgaa gaaaatgctg attcgacaca agatgactca taatcccaat
     1741 cgtcccctgg cagaatgcca gttttgccat aagaagttta caaggaatga ctacctcaaa
     1801 gtgcacatgg acaatatcca tggtgtagct gacagcttgc caactttctt gtacaaagtg
     1861 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1921 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1981 gaacgccagg cggagcataa ccgtct
//
