LOCUS       sequence-285765-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3074
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(512..536)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             544..585
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cctcggccat cgaaaggaag
       61 agcctggacc cttcagagga accagtggat gaggtgctgc agatcccccc atccctgctg
      121 acatgcggcg gctgccagca gaacattggg gaccgctact tcctgaaggc catcgaccag
      181 tactggcacg aggactgcct gagctgcgac ctctgtggct gccggctggg tgaggtgggg
      241 cggcgcctct actacaaact gggccggaag ctctgccgga gagactatct caggcttttt
      301 gggcaagacg gtctctgcgc atcctgtgac aagcggattc gtgcctatga gatgacaatg
      361 cgggtgaaag acaaagtgta tcacctggaa tgtttcaagt gcgccgcctg tcagaagcat
      421 ttctgtgtag gtgacagata cctcctcatc aactctgaca tagtgtgcga acaggacatc
      481 tacgagtgga ctaagatcaa tgggatgata tacccaactt tcttgtacaa agtggttgat
      541 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
      601 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgattag
      661 aggcccggtt tcatattcat
//
