LOCUS       sequence-285768-        1613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3075
FEATURES             Location/Qualifiers
     source          1..1613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1445..1469)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1477..1518
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga atgatttcgg aatcaagaat
       61 atggaccagg tagcccctgt ggctaacagt tacagaggga cactcaagcg ccagccagcc
      121 tttgacacct ttgatgggtc cctgtttgct gtttttcctt ctctaaatga agagcaaaca
      181 ctgcaagaag tgccaacagg cttggattcc atttctcatg actccgccaa ctgtgaattg
      241 cctttgttaa ccccgtgcag caaggctgtg atgagtcaag ccttaaaagc taccttcagt
      301 ggcttcaaaa aggaacagcg gcgcctgggc attccaaaga acccctggct gtggagtgag
      361 caacaggtat gccagtggct tctctgggcc accaatgagt tcagtctggt gaacgtgaat
      421 ctgcagaggt tcggcatgaa tggccagatg ctgtgtaacc ttggcaagga acgctttctg
      481 gagctggcac ctgactttgt gggtgacatt ctctgggaac atctggagca aatgatcaaa
      541 gaaaaccaag aaaagacaga agatcaatat gaagaaaatt cacacctcac ctccgttcct
      601 cattggatta acagcaatac attaggtttt ggcacagagc aggcgcccta tggaatgcag
      661 acacagaatt accccaaagg cggcctcctg gacagcatgt gtccggcctc cacacccagc
      721 gtactcagct ctgagcagga gtttcagatg ttccccaagt ctcggctcag ctccgtcagc
      781 gtcacctact gctctgtcag tcaggacttc ccaggcagca acttgaattt gctcaccaac
      841 aattctggga cgcccaaaga ccacgactcc cctgagaacg gtgcggacag cttcgagagc
      901 tcagactccc tcctccagtc ctggaacagc cagtcgtcct tgctggatgt gcaacgggtt
      961 ccttccttcg agagcttcga agatgactgc agccagtctc tctgcctcaa taagccaacc
     1021 atgtctttca aggattacat ccaagagagg agtgacccgg tggagcaagg caaaccagtt
     1081 atacctgcag ctgtgctggc cggcttcaca ggaagtggac ctattcagct gtggcagttt
     1141 ctcctggagc tgctatcaga caaatcctgc cagtcattca tcagctggac tggagacgga
     1201 tgggagttta agctcgccga ccccgatgag gtggcccgcc ggtggggaaa gaggaaaaat
     1261 aagcccaaga tgaactacga gaagctgagc cggggcttac gctactatta cgacaagaac
     1321 atcatccaca agacgtcggg gaagcgctac gtgtaccgct tcgtgtgcga cctccagaac
     1381 ttgctggggt tcacgcccga ggaactgcac gccatcctgg gcgtccagcc cgacacggag
     1441 gactgcccat ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1501 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1561 tggctttata tatcttgtgg aaaggacgat ggccaactac gctcagccga tga
//
