LOCUS       sequence-285774-        3059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3077
FEATURES             Location/Qualifiers
     source          1..3059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2891..2915)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2923..2964
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga ctgactccaa gtatttcaca
       61 accaataaaa aaggagaaat atttgaacta aaagctgaac tcaacaatga aaagaaagaa
      121 aagagaaagg aggctgtgaa gaaagtgatt gctgctatga ccgtggggaa ggatgttagt
      181 tctctctttc cagacgtagt gaactgtatg cagactgaca atctggaact aaagaagctt
      241 gtgtatctct acttgatgaa ctacgccaag agtcagccag acatggccat catggctgta
      301 aacagctttg tgaaggactg tgaagatcct aatcctttga ttcgagcctt ggcagtcaga
      361 accatggggt gcatccgggt agacaaaatt acagaatatc tctgtgagcc gctccgcaag
      421 tgcttgaagg atgaggatcc ctatgttcgg aaaacagcag cagtctgcgt ggcaaaactc
      481 catgatatca atgcccaaat ggtggaagat cagggatttc tggattctct acgggatctc
      541 atagcagatt caaatccaat ggtggtggct aatgccgtag cggcattatc tgaaatcagt
      601 gagtctcacc caaacagcaa cttacttgat ctgaacccac agaacattaa taagctgctg
      661 acagccctga atgaatgcac tgaatggggc cagattttca tcctggactg cctgtctaat
      721 tacaacccta aagatgatcg ggaggctcag agcatctgtg agcgggtaac tccccggcta
      781 tcccatgcca actcagcagt ggtgctttca gcggtaaaag tcctaatgaa gtttctagaa
      841 ttgttaccta aggattctga ctactacaat atgctgctga agaagttagc ccctccactt
      901 gtcactttgc tgtctgggga gccagaagtg cagtatgtcg ccctgaggaa catcaactta
      961 attgtccaga aaaggcctga aatcttgaag caggaaatca aagtcttctt tgtgaagtac
     1021 aatgatccca tctatgttaa actagagaag ttggacatca tgattcgttt ggcatctcaa
     1081 gccaacattg ctcaggttct ggcagaactg aaagaatatg ctacagaggt ggatgttgac
     1141 tttgttcgaa aagctgtgcg ggccattgga cggtgtgcca tcaaggtgga gcaatctgca
     1201 gagcgctgtg taagcacatt gcttgatcta atccagacca aagtgaatta tgtggtccaa
     1261 gaagcaattg ttgtcatcag ggacatcttc cgcaaatacc ccaacaagta tgaaagtatc
     1321 atcgccactc tgtgtgagaa cttagactcg ctggatgagc cagatgctcg agcagctatg
     1381 atttggattg tgggagaata tgctgaaaga attgacaatg cagatgagtt actagaaagc
     1441 ttcctggagg gttttcacga tgaaagcacc caggtgcagc tcactctgct tactgccata
     1501 gtgaagctgt ttctcaagaa accatcagaa acacaggagc tagtccagca ggtcttgagt
     1561 ttggcaacac aggattctga taatcctgac cttcgagacc ggggctatat ttattggcgc
     1621 cttctctcaa ctgaccctgt tacagctaaa gaagtagtct tgtctgagaa gccactgatc
     1681 tctgaggaga cggaccttat tgagccaact ctgctggatg agctaatctg ccacattggt
     1741 tctttggcct ctgtgtatca taagcctccc aatgcttttg tggaaggaag tcatggaatt
     1801 catcgtaaac acttgccaat tcatcatggg agcactgatg caggtgacag ccctgttggc
     1861 actaccactg caacgaacct ggaacagcct caggttatcc cctctcaagg tgatcttcta
     1921 ggggatcttt taaaccttga cctcggtccc ccagtcaatg tgccacaggt gtcctccatg
     1981 cagatgggag cagtggatct cctaggagga ggactagata gtctgcttgg cagtgacctt
     2041 ggcgggggca ttggaggaag tccggcagtg ggacaatcct tcatcccatc atcggtccct
     2101 gcaacctttg ctccttcacc tacacctgct gtggtcagca gtggactgaa tgacctgttt
     2161 gaactctcca cagggatagg catggcacct ggtggatatg tggctcctaa ggctgtctgg
     2221 ctacctgcag taaaggctaa aggcttggag atttccggaa catttactca ccgccaaggg
     2281 cacatctata tggaaatgaa cttcaccaat aaagctctgc agcacatgac agattttgca
     2341 atccagttta acaaaaatag ctttggtgtc atccccagca ctcctctggc catccataca
     2401 ccactgatgc caaaccagag cattgatgtc tccctgcctc tcaatacctt gggcccagtc
     2461 atgaagatgg aacctctgaa taacctccag gtggctgtga aaaacaatat cgatgtcttc
     2521 tacttcagct gcctcatccc actcaatgtg ctttttgtag aagatggcaa aatggagcgc
     2581 caggtcttcc ttgcaacatg gaaggatatt cccaatgaaa atgaacttca gtttcagatt
     2641 aaggaatgtc atttaaatgc tgacactgtt tccagcaagt tgcaaaacaa caatgtttat
     2701 actattgcca agaggaatgt ggaagggcag gacatgctgt accaatccct gaagctcact
     2761 aatggcattt ggattttggc cgaactacgt atccagccag gaaaccccaa ttacacgctg
     2821 tcactgaagt gtagagctcc tgaagtctct caatacatct atcaggtcta cgacagcatt
     2881 ttgaaaaact acccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     2941 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     3001 atttcttggc tttatatatc ttgtggaaag gacgacgagc actcccatag aaccccgcg
//
