LOCUS       sequence-285777-        1214 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3078
FEATURES             Location/Qualifiers
     source          1..1214
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1046..1070)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1078..1119
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agagaaaaat aagcagaatc
       61 caccttgttt ctgaacccag tataactcat tttctacaag tatcttggga gaaaacactg
      121 gaatctggtt ttgttattac acttactgat ggtcattcag catggactgg gacagtttct
      181 gaatcagaga tttcccaaga agctgatgac atggcaatgg aaaaagggaa atatgttggt
      241 gaactgagaa aagcattgtt gtcaggagca ggaccagctg atgtatacac gtttaatttt
      301 tctaaagagt cttgttattt cttctttgag aaaaacctga aagatgtctc attcagactt
      361 ggttccttca acctagagaa agttgaaaac ccagctgaag tcattagaga acttatttgt
      421 tattgcttgg acaccattgc agaaaatcaa gccaaaaatg agcacctgca gaaagaaaat
      481 gaaaggcttc tgagagattg gaatgatgtt caaggacgat ttgaaaaatg tgtgagtgct
      541 aaggaagctt tggagactga tctttataag cggtttattc tggtgttgaa tgagaagaaa
      601 acaaaaatca gaagtttgca taataaatta ttaaatgcag ctcaagaacg agaaaaggac
      661 atcaaacaag aaggggaaac tgcaatctgt tctgaaatga ctgctgaccg agatccagtc
      721 tatgatgaga gtactgatga ggaaagtgaa aaccaaactg atctctctgg gttggcttca
      781 gctgctgtaa gtaaagatga ttccattatt tcaagtcttg atgtcactga tattgcacca
      841 agtagaaaaa ggagacagcg aatgcaaaga aatcttggga cagaacctaa aatggctcct
      901 caggagaatc agcttcaaga aaaggaaaat tctaggcctg attcttcact acctgagacg
      961 tctaaaaagg agcacatctc agctgaaaac atgtctttag aaactctgag aaacagcagc
     1021 ccagaagacc tctttgatga gatttaccca actttcttgt acaaagtggt tgatatcggt
     1081 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     1141 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga gacgatgtct
     1201 aactcgctcg gatc
//
