LOCUS       sequence-285780-        1421 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3079
FEATURES             Location/Qualifiers
     source          1..1421
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1253..1277)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1285..1326
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgactcacat gataactcac
       61 attggagaga aaacttctga ggataatcag agtggaaaag ccttaagaaa gaactttcct
      121 catagttttt acaagaaaag tcatgctgag gggaaaatgc ctaagtgtgt taaacatgaa
      181 aaagccttca accagtttcc aaatcttact aggcagaata aaactcacac acaagagaaa
      241 ttgtgtgaat gcaaagactg ttggagaact tttcttaatc agtcatccct taagttacat
      301 ataagatctc acaatggaga caaacactat gtatgtaagg aatgtgggaa agccttcagt
      361 aattcctcac accttatagg acatggaaga attcacagtg gagagaagcc ctatgtctgt
      421 aaagaatgtg gtaaagcttt cactcaatcc acaggactta aattacacat cagaactcac
      481 agtggagaaa aaccatataa atgtaaagag tgtgggaaag ccttcaccca ttcttcatac
      541 cttactgatc atacaagaat ccacagtgga aagaagccct atgtatgtat ggaatgtgga
      601 aaagccttca ctagatccac aggacttatt ttacacatgc gaattcacac tggagaaaag
      661 ccatatgaat gtaaggagtg tggaaaagct tttattcatt cctcatacct tacaaaacat
      721 gtaaggattc acagtggaga gaagctgtat ttatgtaagg catgtgggaa agcttttact
      781 cgttcctcag gacttgtttt acacatgaga acacatactg gagaaaagcc ctatgaatgt
      841 aaagaatgtg ggaaagcctt taataattcc tcaatgctta gtcaacatgt aaggattcac
      901 actggagaga agccatatga atgcaaagaa tgtgggaaag ctttcactca atcctcgggc
      961 cttagtaccc atttaagaac tcacactgga gaaaaggcct gtgaatgtaa ggaatgcggt
     1021 aaagcatttg ctcgttccac aaatcttaat atgcacatgc gaacgcacac aggagaaaag
     1081 ccttatgcat gtaaagaatg tgggaaagcc ttcaggtatt ccacatacct taacgttcac
     1141 acacgaactc acactggagc aaaaccatat gaatgtaaga aatgtgggaa aaacttcact
     1201 caatcttcag cacttgctaa acatctaaga actaaggcat gtgaaaaaac cttgccaact
     1261 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1321 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1381 tcttgtggaa aggacgacat acttcgtctt atgcaaggat g
//
