LOCUS       sequence-285783-        1631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3080
FEATURES             Location/Qualifiers
     source          1..1631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1463..1487)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1495..1536
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cccgccgcaa ggccggcagc
       61 gcgccccgcc gagtagagcc cgcgcccgcc gccaacccag acgacgagat ggaaatgcag
      121 gacctcgtca tcgaactcaa gcccgagcca gacgcgcagc cccaacaggc cccaaggctg
      181 gggcccttct ccccgaagga ggtgtcctcg gcggggcggt tcggcggcga accccaccac
      241 tcccctggcc ccatgcccgc cggggccgcc ctcctcgccc tcggcccgcg gaacccgtgg
      301 accctgtgga cgccgttgac cccgaactat cccgaccgcc agccctggac cgacaaacac
      361 ccagatctgt tgacctgcgg ccgctgcctg cagaccttcc cgttggaggc catcactgcc
      421 ttcatggacc acaagaagct gggctgtcag ctcttcagag gccccagccg cggccagggc
      481 tcagaacgag aggagctgaa ggccttgagc tgcctgcgct gtggcaaaca gttcacagtg
      541 gcctggaagc tgctgcgtca cgcccagtgg gaccacggac tgtccatcta ccagacagaa
      601 tcagaggccc cggaggcccc gctcctgggc ctggccgagg tggctgcagc cgtgtcggca
      661 gtggtggggc cagcagctga ggccaagagc ccccgtgcaa gtggcagcgg cctcacccgg
      721 cggagcccca cctgtcctgt gtgcaagaag accctcagct ccttcagcaa cctcaaagtg
      781 cacatgcgct cacacacagg cgagcggccc tatgcttgcg accagtgtcc ctacgcctgc
      841 gcccagagca gcaagctcaa ccgccacaag aagacccacc ggcaggtgcc gccccagagc
      901 cccctcatgg ccgacaccag ccaggagcag gcctctgcag cccctccgga gccggctgtc
      961 catgctgctg cccccaccag cacccttcca tgcagcggtg gtgagggggc tggagccgcc
     1021 gccacagcag gtgtccagga acccggggct cctggcagtg gggctcaagc cggccctggt
     1081 ggagacactt ggggagccat caccacggaa caaagaactg accctgcaaa cagccagaag
     1141 gcatcaccca aaaagatgcc caagtcaggg ggcaagagcc gcgggcccgg gggcagctgt
     1201 gagttctgcg ggaagcattt taccaacagc agcaacctga cggtgcaccg gcgctcacac
     1261 accggggagc gcccctacac ctgtgagttc tgcaactacg cctgcgccca gagcagtaag
     1321 ctcaaccgcc accgccgcat gcacggcatg acgcctggca gcacccgctt cgagtgcccc
     1381 cactgccatg tgcccttcgg cctgcgagcc accctggaca aacacctgcg gcagaagcac
     1441 cctgaggcgg ccggcgaggc ctgcccaact ttcttgtaca aagtggttga tatcggtaag
     1501 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1561 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacaa cgacgcgagc
     1621 catccgcgaa g
//
