LOCUS       sequence-285786-        1427 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3081
FEATURES             Location/Qualifiers
     source          1..1427
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1259..1283)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1291..1332
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agagtgcaat cacgctgtgg
       61 cagttcctgt tgcagttgct gctggatcag aaacatgagc atttgatctg ctggacctcg
      121 aacgatggtg aattcaagct cctcaaagca gaagaagtgg ccaagctgtg gggactccga
      181 aaaaacaaaa caaatatgaa ctatgataag ctgagcagag ccctgcgata ctattatgac
      241 aagaacatca tcaagaaggt gatcgggcag aagtttgtgt acaagtttgt ctctttcccg
      301 gagatcctga agatggatcc tcacgcggtg gagatcagcc gggagagcct tctgctgcag
      361 gacagcgact gcaaggcgtc tccggagggc cgcgaggccc acaaacacgg cctggccgcc
      421 ctcagaagca cgagccgcaa cgaatacatc cactcaggcc tgtactcgtc cttcaccatt
      481 aattccctgc agaacccacc agacgccttc aaggccatca agacggagaa gctggaggag
      541 ccgcccgaag acagcccccc cgtggaagaa gtcaggactg tgatcaggtt tgtgaccaat
      601 aaaaccgaca agcacgtcac caggccggtg gtgtccctgc cttccacgtc agaggctgcg
      661 gcggcgtccg ccttcctggc ctcgtccgtc tcggccaaga tctcctcttt aatgttgcca
      721 aacgctgcca gtatttcatc cgcctcaccc ttctcatctc ggtccccgtc cctgtccccc
      781 aactcacccc tcccttctga acacagaagc ctcttcctgg aggccgcctg ccatgactcc
      841 gattccctgg agcccttgaa cctgtcatcg ggctccaaga ccaagtctcc atctcttccc
      901 ccaaaggcca aaaaacccaa aggcttggaa atctcagcgc ccccgctggt gctctccggc
      961 accgacatcg gctccatcgc cctcaacagc ccagccctcc cctcgggatc cctcacccca
     1021 gccttcttca ccgcacagac accaaatgga ttgcttctga ctccgagtcc actgctctcc
     1081 agcatacatt tctggagcag ccttagtcca gttgctccgc tgagtcctgc caggctgcaa
     1141 gggccaagca cgctgttcca gttccccaca ctgcttaatg gccacatgcc agtgccaatc
     1201 cccagtctgg acagagctgc ttctccagta ctgctttctt caaactctca gaaatcctgc
     1261 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     1321 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1381 tatatatctt gtggaaagga cgataccaac aaagcgacgg gtccgat
//
