LOCUS       sequence-285789-        1253 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3082
FEATURES             Location/Qualifiers
     source          1..1253
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1085..1109)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1117..1158
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgagcgcagc gttcccgccg
       61 tcgctgatga tgatgcagcg cccgctgggg agtagcaccg ccttcagcat agactcgctg
      121 atcggcagcc cgccgcagcc cagccccggc catttcgtct acaccggcta ccccatgttc
      181 atgccctacc ggccggtagt gctgccgccg ccgccgccgc cgccgcccgc gctgccccag
      241 gccgcgctgc agccagcgct gccgcccgca caccctcacc accagatccc cagcctgccc
      301 acaggcttct gctccagcct ggcgcagggc atggcgctca cctctacgct catggccacg
      361 ctccccggcg gcttctccgc gtcgccccag caccaggagg cggcagcggc ccgcaagttc
      421 gcgccgcagc cgctgcccgg cggcggtaac ttcgacaagg cggaggcgct gcaggctgac
      481 gcggaggacg gcaaaggctt cctggccaaa gagggctcgc tgctcgcctt ctccgcggcc
      541 gagacggtgc aggcttcgct cgtcggggct gtccgagggc aagggaaaga cgagtcaaag
      601 gtggaagacg acccgaaggg caaggaggag agcttctcgc tggagagcga tgtggactac
      661 agctcggatg acaatctgac tggccaggca gctcacaagg aggaagaccc gggccacgcg
      721 ctggaggaga ccccgccgag cagcggcgcc gcgggcagca ccacgtctac gggcaagaac
      781 cggcggcggc ggactgcctt caccagcgag cagctgctgg agctagagaa ggagttccac
      841 tgcaaaaagt acctctcctt gaccgagcgc tcgcagatcg cccacgccct caaactcagc
      901 gaggtgcagg tgaaaatctg gttccagaac cgacgggcca agtggaaacg ggtgaaggca
      961 ggcaatgcca attccaagac aggggagccc tcccggaacc ctaagatcgt cgtccccatc
     1021 cctgtccacg tcagcaggtt cgctatcaga agtcagcatc agcagctaga acaggcccgg
     1081 cccttgccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1141 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1201 tggctttata tatcttgtgg aaaggacgac cagtaatgaa tttcaataaa atc
//
