LOCUS       sequence-285792-        1184 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3083
FEATURES             Location/Qualifiers
     source          1..1184
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1016..1040)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1048..1089
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt accaggatta tcccgggaac
       61 tttgacacct cgtcccgggg cagcagcggc tctcctgcgc acgccgagtc ctactccagc
      121 ggcggcggcg gccagcagaa attccgggta gatatgcctg gctcaggcag tgcattcatc
      181 cccaccatca acgccatcac gaccagccag gacctgcagt ggatggtgca gcccacagtg
      241 atcacctcca tgtccaaccc ataccctcgc tcgcacccct acagccccct gccgggcctg
      301 gcctctgtcc ctggacacat ggccctccca agacctggcg tgatcaagac cattggcacc
      361 accgtgggcc gcaggaggag agatgagcag ctgtctcctg aagaggagga gaagcgtcgc
      421 atccggcggg agaggaacaa gctggctgca gccaagtgcc ggaaccgacg ccgggagctg
      481 acagagaagc tgcaggcgga gacagaggag ctggaggagg agaagtcagg cctgcagaag
      541 gagattgctg agctgcagaa ggagaaggag aagctggagt tcatgttggt ggctcacggc
      601 ccagtgtgca agattagccc cgaggagcgc cgatcgcccc cagcccctgg gctgcagccc
      661 atgcgcagtg ggggtggctc ggtgggcgct gtagtggtga aacaggagcc cctggaagag
      721 gacagcccct cgtcctcgtc ggcggggctg gacaaggccc agcgctctgt catcaagccc
      781 atcagcattg ctgggggctt ctacggtgag gagcccctgc acacccccat cgtggtgacc
      841 tccacacctg ctgtcactcc gggcacctcg aacctcgtct tcacctatcc tagcgtcctg
      901 gagcaggagt cacccgcatc tccctccgaa tcctgctcca aggctcaccg cagaagcagt
      961 agcagcgggg accaatcatc agactccttg aactccccca ctctgctggc tctgtaccca
     1021 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     1081 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1141 atatcttgtg gaaaggacga ttcatcctct caacctccag tacc
//
