LOCUS       sequence-285795-        1220 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3084
FEATURES             Location/Qualifiers
     source          1..1220
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1052..1076)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1084..1125
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga ccaccaccct cgtgtctgcc
       61 accatcttcg acttgagcga agttttatgc aagggtaaca agatgctcaa ctatagtgct
      121 cccagtgcag ggggttgcct gctggacaga aaggcagtgg gcacccctgc tggtgggggc
      181 ttccctcgga ggcactcagt caccctgccc agctccaagt tccaccagaa ccagctcctc
      241 agcagcctca agggtgagcc agcccccgct ctgagctcgc gagacagccg cttccgagac
      301 cgctccttct cggaaggggg cgagcggctg ctgcccaccc agaagcagcc cgggggcggc
      361 caggtcaact ccagccgcta caagacggag ctgtgccgcc cctttgagga aaacggtgcc
      421 tgtaagtacg gggacaagtg ccagttcgca cacggcatcc acgagctccg cagcctgacc
      481 cgccacccca agtacaagac ggagctgtgc cgcaccttcc acaccatcgg cttttgcccc
      541 tacgggcccc gctgccactt catccacaac gctgaagagc gccgtgccct ggccggggcc
      601 cgggacctct ccgctgaccg tccccgcctc cagcatagct ttagctttgc tgggtttccc
      661 agtgccgctg ccaccgccgc tgccaccggg ctgctggaca gccccacgtc catcacccca
      721 ccccctattc tgagcgccga tgacctcctg ggctcaccta ccctgcccga tggcaccaat
      781 aacccttttg ccttctccag ccaggagctg gcaagcctct ttgcccctag catggggctg
      841 cccgggggtg gctccccgac caccttcctc ttccggccca tgtccgagtc ccctcacatg
      901 tttgactctc cccccagccc tcaggattct ctctcggacc aggagggcta cctgagcagc
      961 tccagcagca gccacagtgg ctcagactcc ccgaccttgg acaactcaag acgcctgccc
     1021 atcttcagca gactttccat ctcagatgac tacccaactt tcttgtacaa agtggttgat
     1081 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1141 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaacgg
     1201 cactctatac ggcctttggt
//
