LOCUS       sequence-285798-        1391 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3085
FEATURES             Location/Qualifiers
     source          1..1391
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1223..1247)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1255..1296
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ccctggtaga tttgggaaag
       61 aagcttttag aagcggcacg agcaggtcaa gatgatgaag ttcgtatttt gatggcaaat
      121 ggagctccct ttactacaga ctggctggga acttctccac ttcatctagc agcacagtat
      181 ggtcattatt ccaccacaga ggtactgctg cgagctggtg tgagcagaga tgccagaacc
      241 aaagtggacc gaacaccatt acatatggca gcttctgagg gccatgccag catagtagag
      301 gttttactta agcatggtgc tgatgtcaat gcaaaggaca tgttaaagat gacagctctc
      361 cattgggcca cagaacacaa tcatcaagag gtggtggaac ttttaatcaa atatggtgct
      421 gatgtacaca cgcaaagtaa attttgtaaa actgcatttg atatttcaat agacaatgga
      481 aatgaagatt tagcagagat attacagatt gctatgcaga accaaatcaa cacaaaccca
      541 gagagtcctg acactgtgac aatacatgct gcaacaccac agtttatcat tggacctgga
      601 ggggtggtga acctaacagg tctggtatct tcagaaaatt catccaaggc aacagatgaa
      661 acgggtgtat ctgctgttca gtttggaaac tcttctacat cagtattagc tacattagct
      721 gccttagctg aagcatctgc tccattgtcc aattcttcag aaactccagt agtggccaca
      781 gaagaagtag ttactgcaga atctgtggat ggtgccattc agcaagtagt tagttcaggg
      841 ggtcagcaag tcatcacaat agttacagat ggaattcagc ttggaaattt gcactctatt
      901 ccaaccagtg gaattggtca gcccatcatt gtgaccatgc cagatggaca acaagtatta
      961 acagtaccag caacagacat tgctgaagaa actgttataa gtgaagaacc accagctaag
     1021 agacaatgta tcgaaataat tgaaaaccgg gtggaatctg cagaaataga agagagagaa
     1081 gctcttcaga aacagctgga tgaagcaaat cgagaagcac aaaaatatcg acagcagctc
     1141 ctaaagaaag aacaggaagc agaggcctac agacagaagt tggaagctat gactcgtctt
     1201 cagactaata aagaagctgt ttacccaact ttcttgtaca aagtggttga tatcggtaag
     1261 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1321 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacgg aacttggctg
     1381 gccaaaccga t
//
