LOCUS       sequence-28580-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Lau P, Verrier JD, Nielsen JA, Johnson KR, Notterpek L, Hudson LD
  TITLE     Identification of dynamically regulated microRNA and mRNA networks 
            in developing oligodendrocytes.
  JOURNAL   J Neurosci. 2008 Nov 5. 28(45):11720-30.
  PUBMED    18987208
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2008 Nov 5. 28(45):11720-30."
COMMENT     Sequence Label: pDsRed-Sensor
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..709
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVXQYERAEGRHH
                     LFL"
     primer_bind     complement(165..183)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(215..235)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     433..452
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     627..650
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcggatccgc caccatggcc tcctccgagg acgtcatcaa
       61 ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg aacggccacg agttcgagat
      121 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      181 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc agtacggctc
      241 caaggtgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt ccttccccga
      301 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
      361 ggactcctcc ctgcaggacg gctccttcat ctacaaggtg aagttcatcg gcgtgaactt
      421 cccctccgac ggccccgtaa tgcagaagaa gactatgggc tgggaggcct ccaccgagcg
      481 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac aaggccctga agctgaagga
      541 cggcggccac tacctggtgg agttcaagtc catctacatg gccaagaagc ccgtgcagct
      601 gcccggctac tactacgtgg actccaagct ggacatcacc tcccacaacg aggactacac
      661 catcgtggan cagtacgagc gcgccgaggg ccgccaccac ctgttcctgt a
//
