LOCUS       sequence-285801-        2342 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3086
FEATURES             Location/Qualifiers
     source          1..2342
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2174..2198)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2206..2247
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ctcagtggta cgaacttcag
       61 cagcttgact caaaattcct ggagcaggtt caccagcttt atgatgacag ttttcccatg
      121 gaaatcagac agtacctggc acagtggtta gaaaagcaag actgggagca cgctgccaat
      181 gatgtttcat ttgccaccat ccgttttcat gacctcctgt cacagctgga tgatcaatat
      241 agtcgctttt ctttggagaa taacttcttg ctacagcata acataaggaa aagcaagcgt
      301 aatcttcagg ataattttca ggaagaccca atccagatgt ctatgatcat ttacagctgt
      361 ctgaaggaag aaaggaaaat tctggaaaac gcccagagat ttaatcaggc tcagtcgggg
      421 aatattcaga gcacagtgat gttagacaaa cagaaagagc ttgacagtaa agtcagaaat
      481 gtgaaggaca aggttatgtg tatagagcat gaaatcaaga gcctggaaga tttacaagat
      541 gaatatgact tcaaatgcaa aaccttgcag aacagagaac acgagaccaa tggtgtggca
      601 aagagtgatc agaaacaaga acagctgtta ctcaagaaga tgtatttaat gcttgacaat
      661 aagagaaagg aagtagttca caaaataata gagttgctga atgtcactga acttacccag
      721 aatgccctga ttaatgatga actagtggag tggaagcgga gacagcagag cgcctgtatt
      781 ggggggccgc ccaatgcttg cttggatcag ctgcagaact ggttcactat agttgcggag
      841 agtctgcagc aagttcggca gcagcttaaa aagttggagg aattggaaca gaaatacacc
      901 tacgaacatg accctatcac aaaaaacaaa caagtgttat gggaccgcac cttcagtctt
      961 ttccagcagc tcattcagag ctcgtttgtg gtggaaagac agccctgcat gccaacgcac
     1021 cctcagaggc cgctggtctt gaagacaggg gtccagttca ctgtgaagtt gagactgttg
     1081 gtgaaattgc aagagctgaa ttataatttg aaagtcaaag tcttatttga taaagatgtg
     1141 aatgagagaa atacagtaaa aggatttagg aagttcaaca ttttgggcac gcacacaaaa
     1201 gtgatgaaca tggaggagtc caccaatggc agtctggcgg ctgaatttcg gcacctgcaa
     1261 ttgaaagaac agaaaaatgc tggcaccaga acgaatgagg gtcctctcat cgttactgaa
     1321 gagcttcact cccttagttt tgaaacccaa ttgtgccagc ctggtttggt aattgacctc
     1381 gagacgacct ctctgcccgt tgtggtgatc tccaacgtca gccagctccc gagcggttgg
     1441 gcctccatcc tttggtacaa catgctggtg gcggaaccca ggaatctgtc cttcttcctg
     1501 actccaccat gtgcacgatg ggctcagctt tcagaagtgc tgagttggca gttttcttct
     1561 gtcaccaaaa gaggtctcaa tgtggaccag ctgaacatgt tgggagagaa gcttcttggt
     1621 cctaacgcca gccccgatgg tctcattccg tggacgaggt tttgtaagga aaatataaat
     1681 gataaaaatt ttcccttctg gctttggatt gaaagcatcc tagaactcat taaaaaacac
     1741 ctgctccctc tctggaatga tgggtgcatc atgggcttca tcagcaagga gcgagagcgt
     1801 gccctgttga aggaccagca gccggggacc ttcctgctgc ggttcagtga gagctcccgg
     1861 gaaggggcca tcacattcac atgggtggag cggtcccaga acggaggcga acctgacttc
     1921 catgcggttg aaccctacac gaagaaagaa ctttctgctg ttactttccc tgacatcatt
     1981 cgcaattaca aagtcatggc tgctgagaat attcctgaga atcccctgaa gtatctgtat
     2041 ccaaatattg acaaagacca tgcctttgga aagtattact ccaggccaaa ggaagcacca
     2101 gagccaatgg aacttgatgg ccctaaagga actggatata tcaagactga gttgatttct
     2161 gtgtctgaag tgtacccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     2221 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     2281 tcgatttctt ggctttatat atcttgtgga aaggacgagc acttcaacgc acacgcccat
     2341 tc
//
