LOCUS       sequence-285804-         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3087
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(530..554)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             562..603
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ctgtggatcc cctatccagc
       61 aaagctctaa agatcaagcg agagctgagc gagaacacgc cgcacctgtc ggacgaggcg
      121 ctgatggggc tgtcggtgcg cgagctgaac cggcatctgc gcgggctctc cgccgaggag
      181 gtgacacggc tcaagcagcg gcgccgcaca ctcaaaaacc gtggctacgc cgccagctgc
      241 cgcgtgaagc gcgtgtgcca gaaggaggag ctgcagaagc agaagtcgga gctggagcgc
      301 gaggtggaca agctggcgcg cgagaacgcc gccatgcgcc tggagctcga cgcgctgcgc
      361 ggcaagtgcg aggcgctgca gggcttcgcg cgctccgtgg ccgccgcccg cgggcccgcc
      421 acgctcgtgg cgccggccag cgtcatcacc atcgtcaagt ccaccccggg ctcggggtct
      481 ggccccgccc acggcccgga ccccgcccac ggcccggcct cctgctccta cccaactttc
      541 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
      601 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      661 tgtggaaagg acgaaggtat ctctacgggc gacgcctt
//
