LOCUS       sequence-285810-        1388 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3089
FEATURES             Location/Qualifiers
     source          1..1388
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1220..1244)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1252..1293
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga atacaaactc aaaggaggtt
       61 ttatccctgg gtgttcaagt tcccgaggca tgggaagaac ttctgacaat gaaagtggaa
      121 gcaaaaagtc accttcaatg gcaggaatcc agactgaaac gcagtaatcc actggcaagg
      181 gaaatcttcc gaaggcactt tcgacagctg tgctaccaag agacccctgg accaagggag
      241 gctcttactc gactccagga actttgctac cagtggttga ggccacatgt gagcacaaag
      301 gagcagattt tggatctgct ggtgctggag cagtttctat ccattctgcc caaggagctc
      361 cagggctggg tgagggaaca ctgtccagag agtggagaag aggctgtgat tttgctggag
      421 gatctggaga gagagctcga tgaaccacaa catgagatgg tggcccacag acacagacaa
      481 gaagtcctct gtaaagagat ggtgcctcta gcagagcaga caccactgac ccttcagtcc
      541 cagcctaagg agccacagct cacatgtgac tctgctcaga agtgccattc tattggagag
      601 acagatgaag taaccaagac tgaggacaga gagttggtgc taaggaaaga ctgtcctaag
      661 atagtggaac cacatgggaa aatgtttaat gagcagacct gggaggtatc acagcaggat
      721 ccctcacatg gagaagttgg tgaacataag gataggatag agaggcagtg gggaaacctc
      781 ttaggagagg ggcaacacaa atgtgatgaa tgtgggaaga gctttactca gagctcaggt
      841 ctcattcgac atcaaagaat tcatactgga gaaagacctt atgaatgtaa tgaatgtggg
      901 aaagccttca gtcgaagttc tggtcttttt aatcaccgag gaatccacaa tatacagaaa
      961 cggtaccact gcaaggagtg tgggaaggtc ttcagtcaga gtgcgggtct tatccagcat
     1021 cagagaatcc acaaaggaga aaagccgtat cagtgcagcc agtgcagtaa gagctacagt
     1081 cggcgttcat ttctcattga acatcagaga agccacacag gggagcgacc tcaccagtgc
     1141 attgaatgtg ggaaaagctt taatcgacac tgcaacctca ttcgccatca gaagatccac
     1201 acagtggctg agctggtcta cccaactttc ttgtacaaag tggttgatat cggtaagcct
     1261 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     1321 agtatttcga tttcttggct ttatatatct tgtggaaagg acgactgtgt taggacgttt
     1381 gtcagcat
//
