LOCUS       sequence-285813-        1445 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3090
FEATURES             Location/Qualifiers
     source          1..1445
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1277..1301)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1309..1350
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgataggcat gttggaaagt
       61 cttcagcatg aatcagatct ccttcagcat gatcaaattc atactggaga gaaaccttat
      121 gaatgtaatg aatgtagaaa aacttttagc ctgaagcaaa accttgtaga gcataagaaa
      181 atgcatactg gagagaaatc tcatgaatgc actgaatgtg gtaaagtgtg ctctcgagtc
      241 tcatctctta ctctacacct tagaagtcac acaggaaaga aggcatataa atgtaataaa
      301 tgtggaaaag ccttcagcca gaaggaaaac ttcctttctc atcagaaaca tcacactgga
      361 gagaaacctt atgaatgtga aaaagtttct attcagatgc caaccatcat cagacaccag
      421 aaaaatcata caggaaccaa accctatgca tgtaaggaat gtggcaaagc ctttaacggc
      481 aaagcatatc tcactgagca tgagaaaatt catactggag aaaaaccatt tgaatgtaat
      541 cagtgtggaa gagccttcag ccagaagcaa tacctcatta aacatcagaa catccatact
      601 ggaaagaagc cctttaaatg tagtgagtgt ggaaaagctt ttagccagaa ggaaaacctc
      661 attatacatc agagaatcca tactggagag aaaccttatg aatgtaaagg gtgtgggaaa
      721 gctttcattc agaagtcaag cctcattaga caccagagaa gtcacacagg agagaaacct
      781 tatacgtgta aggaatgtgg gaaagccttc agtggcaagt caaatctcac agagcatgag
      841 aaaattcata ttggagagaa accctacaaa tgtaatgaat gtggaacaat cttcaggcag
      901 aagcaatacc tcattaaaca tcacaatatt catacaggag agaaacccta tgaatgtaat
      961 aaatgtggaa aagccttctc tcgaatcaca tcacttattg tacatgtgag aattcataca
     1021 ggtgataagc cttatgagtg taaggtctgt gggaaagcct tctgtcaaag ctcatctctt
     1081 actgtgcaca tgagaagcca tacaggtgag aaaccctatg gttgtaatga atgtgggaaa
     1141 gccttttctc agttctcaac ccttgctctg cacatgagaa tccatactgg tgaaaaacct
     1201 tatcagtgta gtgaatgtgg gaaagctttc agccaaaagt cacatcacat tagacaccag
     1261 agaattcata ctcatttgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1321 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1381 atttcgattt cttggcttta tatatcttgt ggaaaggacg atgccctaat gtttggacct
     1441 agttt
//
