LOCUS       sequence-285819-        1976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3092
FEATURES             Location/Qualifiers
     source          1..1976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1808..1832)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1840..1881
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggcccccta tcccggcagt
       61 ggcggcggaa gcgaggtcaa atgcgtggga ggccgcggcg ccagcgtccc ctgggacttt
      121 ctacccgggc tgatggtcaa ggcgccgtcc ggaccgtgcc tgcaggcgca gcgcaaggag
      181 aagtcccgga acgcggcgcg ctcgcggcgc gggaaggaga acctggagtt cttcgagctg
      241 gccaagcttc tcccgctgcc cggcgccatc tccagccagc tggacaaggc ttccatcgtg
      301 cgcctcagcg tcacctacct ccgcctgcgc cggttcgccg ctctgggggc gccgccctgg
      361 gggctgagag ccgcggggcc gccagctggc ctcgccccag gccgccgcgg ccccgcagcg
      421 ctggtctccg aagtcttcga gcagcacctg ggaggtcaca tcttgcagtc cctggatggc
      481 tttgtgttcg ccttgaacca ggaaggaaaa ttcctctaca tctcagagac agtctccatc
      541 tatctgggtc tctcacaggt ggagatgacg ggcagcagcg tcttcgacta cattcaccct
      601 ggggaccact cagaggtgct ggagcaactg gggctgcgga cgccgacgcc cggcccccca
      661 accccgccct ccgtctcctc ttcctcctcc tcttcctctt cgcttgcaga tacccccgag
      721 atcgaggcca gcctcaccaa ggtgcccccc tcctccctgg tccaggagcg ctccttcttt
      781 gtccgcatga aatccacgct caccaagagg gggctgcacg tcaaggcctc agggtacaag
      841 gtcatccacg tgactgggcg ccttcgggcc cacgccctgg gccttgtggc cctcgggcac
      901 acgttgcccc cggcccccct ggctgagctg ccactccatg gacacatgat cgtcttccgt
      961 ctcagcctgg gtctcaccat ccttgcttgt gagagcagag tcagcgacca catggacctg
     1021 gggccctcag agctggtggg ccgcagctgc taccagtttg tccacggaca ggacgccacg
     1081 aggatccgcc agagccacgt ggacttgctg gacaagggtc aggtgatgac tggttactac
     1141 cgttggctgc agcgtgccgg gggcttcgtg tggctgcagt ctgtggccac agtggctggg
     1201 agcgggaaga gccccgggga gcaccatgtg ctttgggtca gccacgtgct cagccaagcc
     1261 gagggtggcc aaactccttt ggatgccttc cagcttccag ccagcgtggc ctgtgaggag
     1321 gcatccagcc cggggccaga gcccacagag ccggagcctc cgacggaagg gaagcaggct
     1381 gccccagcgg agaacgaggc cccccagacc cagggcaaac gcatcaaagt ggagcccggc
     1441 ccgagggaaa ccaaaggctc cgaggacagt ggcgacgagg atccctccag ccacccggcc
     1501 acaccgaggc ccgagttcac ctctgtcatc cgggcagggg tcctgaagca ggatccggtg
     1561 cggccatggg gcctggcgcc tcccggggac cccccgccca ccctcctgca cgcgggcttc
     1621 ctgccgccgg tggtgcgggg cctgtgcaca cccggcacca tccgctacgg ccccgcggag
     1681 ctgggcctgg tgtacccgca cctgcagagg ctgggtccgg gccccgcgct cccggaggcc
     1741 ttttacccgc ccctgggcct gccctacccg gggcccgcgg gcaccaggct gccgcggaag
     1801 ggggactgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1861 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1921 tcttggcttt atatatcttg tggaaaggac gaatctcgcg ccaccctaag aatcaa
//
