LOCUS       sequence-285822-        2894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3093
FEATURES             Location/Qualifiers
     source          1..2894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2726..2750)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2758..2799
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga ctgaggaatc agaggagaca
       61 gtcctgtaca ttgagcaccg ctatgtctgc tctgagtgca accagctgta tggatcactg
      121 gaagaggtgc ttatgcacca aaactcccac gtgccccagc agcactttga gctggtgggc
      181 gtggctgatc ccggagtcac tgtggccaca gacacagctt caggcacggg cctctatcag
      241 acccttgtgc aggagagcca gtaccagtgc ctggagtgtg gtcaactgct gatgtcaccc
      301 agccagctcc tggagcacca ggagctgcac ctgaagatga tggcacccca ggaggcagtg
      361 ccagctgagc catcacctaa ggcaccaccc ctgagctcca gcaccatcca ctacgagtgt
      421 gtggattgca aggctctctt tgccagccag gagctctggc tgaaccaccg gcagacgcac
      481 ctccgggcca cacccaccaa ggctcctgcc cctgttgtcc tggggtcccc agttgttcta
      541 gggcctcctg tgggccaggc ccgagtggct gtggagcact cataccgaaa ggcagaagag
      601 ggtggggaag gggcgactgt cccatctgcc gctgccacca ccactgaggt agtgactgag
      661 gtggagctgc tcctctacaa gtgctctgag tgctcccagc tcttccagct gccggcggat
      721 ttcctggagc accaggccac tcacttccct gctcctgtac ccgagtctca ggagcctgcc
      781 ttacagcagg aggtgcaggc ctcgtcacct gcagaggtgc ctgtgtctca gcctgacccc
      841 ttgccagctt ctgaccacag ttacgagctg cgcaatggtg aagccattgg gcgggatcgc
      901 cgggggcgca gggcccggag gaacaacagt ggagaagcag gcggggcagc cacacaggag
      961 ctcttctgct cagcctgtga ccagctcttt ctctcacccc accagctaca gcagcacctg
     1021 cggagtcacc gggagggcgt ctttaagtgc cccctgtgca gtcgtgtctt ccctagccct
     1081 tccagtctgg accagcacct tggagaccat agcagcgagt cacacttcct gtgtgtagac
     1141 tgtggcctgg ccttcggcac agaggccctc ctcctggccc accggcgagc ccacaccccg
     1201 aatcctctgc attcatgtcc atgtgggaag acctttgtca accttaccaa gttcctttat
     1261 caccggcgta ctcatggggt agggggtgtc cctctgccca caacaccagt cccaccagag
     1321 gaacctgtca ttggtttccc tgagccagcc ccagcagaga ctggagagcc agaggcccct
     1381 gagccccctg tgtctgagga gacctcagca gggcccgctg ccccaggcac ctaccgctgc
     1441 ctcctgtgca gccgtgaatt tggaaaggcc ttgcagctga cccggcacca acgttttgtg
     1501 catcggctgg agcggcgcca taaatgcagc atttgtggca agatgttcaa gaagaagtct
     1561 cacgtgcgta accacctgcg cacacacaca ggggagcggc ccttcccctg ccctgactgc
     1621 tccaagccct tcaactcacc tgccaacctg gcccgccacc ggctcacaca cacaggagag
     1681 cggccctacc ggtgtgggga ctgtggcaag gctttcacgc aaagctccac actgaggcag
     1741 caccgcttgg tgcatgccca gcacttcccc taccgctgcc aggaatgtgg ggtgcgtttt
     1801 caccgtcctt accgcctgct catgcaccgc taccatcaca caggtgaata cccctacaag
     1861 tgtcgcgagt gcccccgctc cttcttgctg cgtcggctgc tggaggtgca ccagctcgtg
     1921 gtccatgccg ggcgccagcc ccaccgctgc ccatcctgtg gggctgcctt cccctcctca
     1981 ctgcggctcc gggagcaccg ctgtgcagcc gctgctgccc aggccccacg gcgctttgag
     2041 tgtggcacct gtggcaagaa agtgggctca gctgctcgac tgcaggcaca cgaggcggcc
     2101 catgcagctg ctgggcctgg agaggtcctg gctaaggagc cccctgcccc tcgagcccca
     2161 cgggccactc gtgcaccagt tgcctctcca gcagcccttg gaagcactgc tacagcatcc
     2221 cctgcggccc ctgcccgccg ccggggtcta gagtgcagcg agtgcaagaa gctgttcagc
     2281 acagagacgt cactgcaggt gcaccggcgc atccacacag gtgagcggcc atacccatgt
     2341 ccagactgtg gcaaagcgtt ccgtcagagt acccacctga aagaccaccg gcgcctgcac
     2401 acaggtgagc ggccctttgc ctgtgaagtg tgtggcaagg cctttgccat ctccatgcgc
     2461 ctggcagaac atcgccgcat ccacacaggc gaacgaccct actcctgccc tgactgtggc
     2521 aagagctacc gctccttctc caacctctgg aagcaccgca agacccatca gcagcagcat
     2581 caggcagctg tgcggcagca gctggcagag gcggaggctg ccgttggcct ggccgtcatg
     2641 gagactgctg tggaggcgct acccctggtg gaagccattg agatctaccc tctggccgag
     2701 gctgaggggg tccagatcag tggctgccca actttcttgt acaaagtggt tgatatcggt
     2761 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     2821 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga atacatccct
     2881 gattttaatc acat
//
