LOCUS       sequence-28583-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Estall JL, Ruas JL, Choi CS, Laznik D, Badman M, Maratos-Flier E, 
            Shulman GI, Spiegelman BM
  TITLE     PGC-1alpha negatively regulates hepatic FGF21 expression by 
            modulating the heme/Rev-Erb(alpha) axis.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Dec 29. 106(52):22510-5.
  PUBMED    20018698
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Dec 29. 106(52):22510-5."
COMMENT     Sequence Label: pAdTrack-CMV FLAG Rev-Erb alpha
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(520..543)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(586..685)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(615..635)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attctcattc ttcagacacc ttttgtactg gatgttctgc tggatgctcc gacggaaaaa
       61 gcccttgcag ccctcgcagg cgtgcacacc gtagtggaag cccgaggcaa cgtccccaca
      121 cactttacac agtaacacca tgccattcag cttggtgatg ttgctggtgc tcttgctggg
      181 ggacactcgg ctgctgtcct ccatggccac ttgtagactc ccaggggggc tcccattata
      241 gaaggaggag gaggatgacg acgaggaaga tgaggaagaa ggggagccgt catcactcag
      301 gctgggtgga atgctcccaa aggagcgagc cgggtcttgg gtgagggagc cagtggggga
      361 tggtgggaag taggtgggac agccttgggt cagggactgg aagctgccat tggagttgtc
      421 actatagagg gattcagggc tggtgcggct tggggaggag ccactggagc caatgtaggt
      481 gatgacgcca cctgtgttgt tgttggagtc cagggtggtc ttgtcgtcat cgtctttgta
      541 gtccatggta ccagatctct agcggatctg acggttcact aaaccagctc tgcttatata
      601 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
      661 ttttggaaag tcccgttgat tttggtg
//
