LOCUS       sequence-285834-        1916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3097
FEATURES             Location/Qualifiers
     source          1..1916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1748..1772)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1780..1821
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agagcttggg gctgcacacg
       61 gtgaccctta gtgatgggac aacagcctac gtccagcaag ctgtcaaagg agagaagctt
      121 cttgaagggc aggtgatcca gctcgaggat gggaccaccg catacattca ccaggtgacg
      181 gtacagaaag aagctctctc ctttgaggat ggtcagcctg tgcagctgga agatggcagc
      241 atggcttaca tacaccgcac acccagagaa ggctatgacc ccagcaccct ggaagccgtc
      301 caactggaag atggctccac tgcctacatt caccaccctg tggctgtgcc atcggagagc
      361 accatcctgg ccgtacagac agaggtgggc ttggaggacc tggcagcaga ggatgatgag
      421 ggcttcagtg cagacgcagt ggtggccctg gagcagtatg ccagcaaggt tcttcatgac
      481 agccagattc cccgtaatgg aaaagggcag caagttggag acagagcatt ccgctgtggc
      541 tacaagggct gtgggcgtct ctacaccacc gctcatcact taaaggtgca tgaacgagct
      601 catacaggtg accgtccata cagatgtgac ttccccagct gtggaaaggc ctttgccaca
      661 ggctatggac tgaagagcca cgttcgtacc cacactggtg agaaaccata caagtgccca
      721 gaggagctgt gcagcaaggc cttcaagacc tcaggagacc tgcagaagca tgtccgtacc
      781 cacactggtg aacgcccgtt ccagtgccct tttgagggct gtggccgctc cttcaccaca
      841 tctaacatcc gcaaggtaca tgtgcgcacc cacacaggcg agaggcccta cacctgcccg
      901 gagccccact gtggccgcgg cttcaccagc gccaccaact ataagaatca cgtgcgcatc
      961 cacacagggg agaagccata cgtttgcacg gtgccaggct gcgggaaacg cttcaccgag
     1021 tactcgagct tgtataagca ccacgtggtg cacacacact gcaagcccta cacctgcagc
     1081 acctgcggca agacctaccg gcagacctcc accttggcca tgcacaagcg cagtgcccat
     1141 ggcgagctgg aggccacgga ggagagtgag caggccctct atgagcagca gcaacttgag
     1201 gccgcctctg cagccgagga gagtccgcca cccaaacgac cccggatagc ttacctttcg
     1261 gaggtgaagg aagagagaga tgacatccca gcccaggtgg cgatggtgac tgaagaagat
     1321 ggggcccccc aggtggctct gatcactcag gatggtgccc agcaggtcag cctgtccccg
     1381 gaagacctgc aggccctggg gagtgccatc agtatggtca cccagcacgg cagcaccacc
     1441 ctcaccatcc ccagtcctga tgccgacctg gccacatctg gcacacatac agtcaccatg
     1501 gtcagcgccg atggcaccca gacgcagccc gtcacaatca ttacctctgg ggctgtggtg
     1561 gctgaggact caagtgtagc atctcttcgt catcaacagg tggcactgtt ggccacagcc
     1621 aacggaacgc acattgcagt gcagctggag gaacagcaga ccttagagga ggccatcaat
     1681 gtggccactg cggccatgca gcaaggggct gtgaccctgg agacaacagt gtcggagagt
     1741 ggctgctgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1801 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1861 tcttggcttt atatatcttg tggaaaggac gaggaatgat gaaactacct cacagc
//
