LOCUS       sequence-285837-        1991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3098
FEATURES             Location/Qualifiers
     source          1..1991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1823..1847)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1855..1896
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gagagacctt ggaggccctc
       61 agctccctgg gattctctgt gggacagcca gagatggccc cccaaagtga gcccagggaa
      121 ggatcccata atgcccagga gcagatgtcc tcttctaggg aagagagagc actgggggtg
      181 tgctcagggc acgaggcccc tacaccggag gaaggtgccc acacagaaca agccgaggct
      241 ccctgcagag gccaggcgtg ctcagcacag aaggctcagc ctgtgggtac ctgcccagga
      301 gaggagtgga tgattcggaa ggtgaaggtg gaggacgaag atcaggaggc agaagaggag
      361 gtcgaatggc cccagcatct atcgttactt cccagcccct ttcccgcgcc tgacctgggg
      421 catctggctg ccgcgtacaa actggagcca ggggccccgg gggcactgag tgggctcgcg
      481 ctgtctgggt ggggtccgat gccggagaag ccctacggct gcggggagtg tgagcggcgc
      541 ttccgggacc agctgacgtt gcgactgcac cagcggctgc accggggcga gggcccctgc
      601 gcctgcccgg actgcggccg cagcttcacg cagcgcgccc acatgctact gcatcagcgc
      661 agccaccgcg gcgagcggcc tttcccgtgc tccgagtgcg acaagcgctt cagcaagaag
      721 gcccatctga cccgccacct gcgcacgcac acgggcgagc ggccctaccc gtgcgcggag
      781 tgcggcaagc gcttcagcca gaagatccac ctgggctcgc accaaaagac ccacaccggc
      841 gagcggccct tcccctgcac ggaatgcgag aagcgctttc gcaagaagac gcacttgatt
      901 cggcaccagc gcatccatac gggcgagagg ccctaccagt gcgcacagtg cgcacgcagc
      961 ttcacgcaca agcagcactt ggtgcggcac caaagggtgc accagacggc cggcccggcc
     1021 aggccctctc ccgactcgtc cgcttctcct cattccactg ccccgtcccc gaccccatcc
     1081 tttcccgggc caaagccttt cgcgtgctcc gactgcggct tgagcttcgg ctggaaaaag
     1141 aacctcgcca cgcaccagtg tctgcaccgc agcgagggtc gcccctttgg gtgcgatgag
     1201 tgcgcactgg gcgccaccgt ggatgccccc gccgccaagc ccctggccag cgcgcctggc
     1261 ggaccgggct gcggcccagg atccgatccc gtggtgcccc agcgcgcccc ctcgggcgag
     1321 cggtccttct tctgcccgga ctgcgggcgc ggcttctccc atgggcagca cctggcgcgg
     1381 cacccgcgcg tgcacacggg cgaacggccc ttcgcctgca cgcagtgtga ccgccgcttc
     1441 ggctcgcggc ctaatctggt cgcccactcc agggcccaca gcggcgccag gcctttcgcc
     1501 tgcgctcagt gcggccgccg cttcagccgc aagtcgcacc tgggccgcca ccaggcggtg
     1561 cacactggca gccgccccca cgcctgcgcc gtctgcgccc gcagcttcag ctccaaaacc
     1621 aacctagtcc gccaccaggc gatccacaca ggctcccgcc ccttctcctg cccgcagtgc
     1681 ggaaagagct tcagccgcaa gacccacctg gtgcggcacc agctcattca cggcgaagcc
     1741 gcccacgcgg ccccggacgc cgcccttgcg gccccagcct ggtccgctcc ccccgaggtg
     1801 gcgccgcccc cgctcttctt ctgcccaact ttcttgtaca aagtggttga tatcggtaag
     1861 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1921 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagag gttatgtatt
     1981 ctaggagatg g
//
