LOCUS       sequence-28584-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Estall JL, Ruas JL, Choi CS, Laznik D, Badman M, Maratos-Flier E, 
            Shulman GI, Spiegelman BM
  TITLE     PGC-1alpha negatively regulates hepatic FGF21 expression by 
            modulating the heme/Rev-Erb(alpha) axis.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Dec 29. 106(52):22510-5.
  PUBMED    20018698
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Dec 29. 106(52):22510-5."
COMMENT     Sequence Label: pAdTrack-CMV FLAG Rev-Erb alpha
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(66..269)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(95..115)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        270..573
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(670..689)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     724..743
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcctcgccc ttgctcacca tggtggcgac cggtagcgct agcggatctg acggttcact
       61 aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
      121 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
      181 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
      241 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
      301 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
      361 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
      421 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
      481 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
      541 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
      601 aatgaccccg taattgatta ctattaatca ctagcaganc gtcganaaac ctccgcgtta
      661 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      721 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa ta
//
