LOCUS       sequence-285843-        1691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3100
FEATURES             Location/Qualifiers
     source          1..1691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1523..1547)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1555..1596
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggagtcggc gcctgctcgg
       61 cacaggagaa aacgacgctc cacaccttta acttcttcca cacttccttc acaagcaaca
      121 gagaaaagct cctattttca gaccaccgag atttcactct ggacggtggt ggccgctatt
      181 caggctgtgg agaagaagat ggagtcccag gctgcccggc tacagagcct ggaggggcgc
      241 acggggacag ccgagaagaa gctggctgac tgcgagaaga tggccgtgga gttcgggaac
      301 cagctggagg gcaagtgggc cgtgctgggg accctgctgc aggagtatgg gctactgcag
      361 aggcggctgg agaacgtgga gaacctgctg cgcaacagga acttctggat cctgcggctg
      421 cccccgggca gcaaggggga ggcccccaag gtgtccaggt cactggagaa tgatggcgtc
      481 tgtttcaccg agcaggaatg ggagaatctg gaggattggc agaaggagct ctacagaaac
      541 gtgatggaga gtaactatga gacactggtc tctctgaagg tccttggcca gacagaggga
      601 gaagcggagt tgggtacaga gatgctgggt gacttggaag aggaaggtcc tggtggtgcc
      661 cacccagcag gtggggtcat gatcaaacag gagctacagt atacacagga aggccctgcg
      721 gatcttcctg gagagttctc atgcattgct gaagagcagg ctttcctgag cccagagcag
      781 accgaactct ggggtggtca gggcagttct gtcctcttgg aaacaggtcc tggggactct
      841 actctagagg agcctgttgg tagtagagtt cctagcagca gcagaactgt gggctgcccg
      901 aagcagaaat ctcataggca ggtacagctg gaccaggaat gtgggcaggg cctgaagctg
      961 aaaaaggaca cttcccgccc ctacgaatgt tctgagtgtg agatcacctt ccgctataag
     1021 cagcagctgg ccacacatct gcgcagccac tctgggtggg ggtcttgtac acctgaggag
     1081 ccagaggaga gccttaggcc caggccacgg ctgaaaccac agaccaaaaa ggccaagctg
     1141 catcagtgtg atgtgtgcct gaggagcttc agctgcaagg tgagcctggt gacccatcag
     1201 cgttgccacc tgcaggaggg gcccagtgcc ggccagcatg tccaagagag gttctcaccc
     1261 aacagcctgg ttgccctgcc tggccacatc ccttggagga aaagccggag ttccctcatc
     1321 tgtggttact gtggcaagag cttcagtcac ccatctgact tggtgcggca ccagcgcatc
     1381 cacacgggtg agcggcccta cagctgcact gagtgtgaga agagctttgt ccagaagcag
     1441 cacctcctgc agcaccagaa gatccaccag cgggagcggg gtgggctggc cctggagccc
     1501 ggaaggccca atggcctgct ttacccaact ttcttgtaca aagtggttga tatcggtaag
     1561 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1621 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgattc tatcagtacc
     1681 gactgtttgt c
//
