LOCUS       sequence-285846-        1970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3101
FEATURES             Location/Qualifiers
     source          1..1970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1802..1826)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1834..1875
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gaggccttga gaagaagaag
       61 tatgaacgag gctcggccac caactacatc acccggaaca aagcccggaa gaagctccag
      121 ctgagcttgg ctgactttag gcggctgtgc attctgaagg gcatttatcc ccatgaaccc
      181 aaacacaaga agaaggttaa caagggttct acagcagccc gaacgtttta ccttatcaaa
      241 gacatcaggt ttctcctcca cgaacccatt gtcaacaagt tccgtgaata caaggtgttc
      301 gtccggaagc tccggaaggc ttatgggaag agcgagtgga acactgtaga gcgtttaaag
      361 gacaataagc ccaactacaa actcgaccac atcatcaagg aacggtatcc cacgttcatc
      421 gatgccctgc gggacctgga cgatgccctc tccatgtgct tcctgttttc caccttcccg
      481 cggactggca agtgccacgt gcagaccatt cagctgtgcc gccggctcac tgtggagttc
      541 atgcactaca ttatcgctgc ccgtgccctg cgcaaggtct tcctgtccat caaaggcatt
      601 tactaccagg ccgaggtact ggggcagccc atcgtgtgga tcactcccta tgccttctcc
      661 catgaccacc cgacagacgt ggactacagg gtcatggcca ccttcaccga gttctacacc
      721 acgctgctgg gctttgtcaa cttccgcctt taccagttgc tcaacctcca ctatcccccg
      781 aagctcgagg gtcaggccca agcagaggca aaggccggtg agggcaccta cgcgttggac
      841 tccgagagtt gtatggagaa actggcagcc ctcagtgcca gcctggcccg cgtggtggtg
      901 cctgccacag aggaggaggc cgaggtggat gagtttccca ccgatgggga gatgtcagcg
      961 caggaggaag accgcaggaa ggagctggag gcgcaggaga agcacaagaa gctttttgag
     1021 ggcctgaagt tcttcctgaa ccgagaggtg ccccgtgagg ccctggcctt catcatcagg
     1081 agttttggtg gggaagtgtc ctgggacaaa tctttgtgca ttggggccac ctatgacgtc
     1141 acagactccc gcatcaccca tcagattgtc gaccggcctg ggcagcagac ctcagtcatt
     1201 ggcaggtgct acgtgcagcc ccagtgggtg tttgactcag tgaacgccag gctccttctc
     1261 cccgtggcag agtacttctc tggggtgcag ctgcccccac acctttcacc ctttgtgacc
     1321 gagaaggaag gagattacgt tccacctgag aagctgaagc tgctggctct gcagcgggga
     1381 gaggacccag gaaacctgaa tgagtcagaa gaggaggagg aagaggacga caacaacgaa
     1441 ggtgatggtg atgaagaggg agaaaatgag gaggaggagg aagatgcaga ggctggttca
     1501 gaaaaggagg aagaggcccg gctggcagcc ctggaagagc agaggatgga ggggaagaag
     1561 cccagggtga tggcaggcac cttgaagctg gaggataagc agcggctggc ccaggaggag
     1621 gagagtgagg ccaagcgcct ggccattatg atgatgaaga agcgggagaa gtacctgtac
     1681 cagaagatca tgtttggcaa gaggcgaaaa atccgagagg ccaacaagct ggcggagaag
     1741 cggaaagccc acgatgaggc ggtgaggtct gagaagaagg ccaagaaggc aaggccggag
     1801 tgcccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1861 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1921 ctttatatat cttgtggaaa ggacgagctg ctaaccagga ctggcgtctt
//
