LOCUS       sequence-285849-        1706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3102
FEATURES             Location/Qualifiers
     source          1..1706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1538..1562)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1570..1611
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgccaccttc ggctccgctc
       61 cctgcgcagg gaccaggaaa ggccagaccc agtcggaaaa ggggcaggag gccgagggct
      121 ctgaagttcg tggacgtggc cgtgtacttc tccccggagg agtggggctg cctgcggccc
      181 gcgcagaggg ccctgtaccg ggacgtgatg cgggagacct acggtcacct gggcgcgctc
      241 gggtgcgcag gtcccaaacc agccctcatc tcctggttgg aacgaaacac cgatgactgg
      301 gaaccggctg ctctagatcc gcaggagtac ccgagagggc taacagtcca gagaaaaagc
      361 agaaccagaa agaagaatgg ggagaaggaa gtattcccgc ctaaggaggc accccgaaag
      421 gggaagcgag gccggaggcc cagcaaaccc cgactgattc ctaggcagac gtccgggggc
      481 cccatctgcc ctgactgcgg ctgtaccttc cctgatcatc aggccctgga gagccacaag
      541 tgcgcccaga atctaaaaaa gccttaccct tgcccagact gtgggcgccg cttttcctat
      601 ccatccctgc tggtcagtca ccggcgggca cactccggcg agtgccccta tgtttgtgac
      661 cagtgtggca aacgtttctc ccagcgcaag aacctctccc agcaccaggt catccataca
      721 ggggagaagc cctatcactg ccctgactgt ggtcgctgct tccggaggag ccggtccttg
      781 gccaatcacc ggaccacaca cacaggtgaa aaaccccacc agtgccctag ctgtggacgt
      841 cgcttcgcct acccctccct gctagccatc caccagcgta cacacacggg agagaagccc
      901 tacacttgcc tcgagtgcaa ccgccgcttc cgccagcgca cggccctcgt catccaccag
      961 cgcatccaca cgggcgagaa gccctacccg tgcccggact gcgagcggcg cttctcctcc
     1021 tcctctcgcc tggtcagtca ccggcgtgtg cactctgggg agcgtcccta tgcctgcgag
     1081 cactgtgagg cccgcttctc ccagcgcagc acgctgctcc agcaccagct cttgcacacc
     1141 ggagagaagc cctacccctg cccagactgt gggcgtgcct tccggcggag cggctccctg
     1201 gccatccatc gcagcacgca cacagaggag aagctgcacg cctgcgacga ctgtggtcgc
     1261 cgctttgcct acccctcact gctggccagc caccggcgcg tgcactcggg cgagcggccc
     1321 tatgcctgcg acctttgctc caagcgtttt gctcagtgga gccacctggc ccagcaccag
     1381 ctgctgcaca cgggggagaa gcctttcccc tgcctcgagt gtggccggtg cttccgccag
     1441 aggtggtctc tggctgtcca caagtgtagc cccaaggccc caaactgtag ccctagatct
     1501 gctatcgggg gctccagtca gaggggcaac gcccattacc caactttctt gtacaaagtg
     1561 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1621 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1681 gatttggcat cccccctgta gtgcgc
//
