LOCUS       sequence-285852-        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3103
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             202..225
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 accgccacca tggacaatgc aagaatgaac tccttcctgg aataccccat acttagcagt
       61 ggcgactcgg ggacctgctc agcccgagcc tacccctcgg accataggat tacaactttc
      121 cagtcgtgcg cggtcagcgc caacagttgc ggcggcgacg accgcttcct agtgggcagg
      181 ggggtgcaga tcggttcgcc ccaccaccac caccaccacc accatcgcca cccccagccg
      241 gctacctacc agacttccgg gaacctgggg gtgtcctact cccactcaag ttgtggtcca
      301 agctatggct cacagaactt cagtgcgcct tacagcccct acgcgttaaa tcaggaagca
      361 gacgtaagtg gtgggtaccc ccagtgcgct cccgctgttt actctggaaa tctctcatct
      421 cccatggtcc agcatcacca ccaccaccag ggttatgctg ggggcgcggt gggctcgcct
      481 caatacattc accactcata tggacaggag caccagagcc tggccctggc tacgtataat
      541 aactccttgt cccctctcca cgccagccac caagaagcct gtcgctcccc cgcatcggag
      601 acatcttctc cagcgcagac ttttgactgg atgaaagtca aaagaaaccc tcccaaaaca
      661 gggaaagttg gagagtacgg ctacctgggt caacccaacg cggtgcgcac caacttcact
      721 accaagcagc tcacggaact ggagaaggag ttccacttca acaagtacct gacgcgcgcc
      781 cgcagggtgg agatcgctgc atccctgcag ctcaacgaga cccaagtgaa gatctggttc
      841 cagaaccgcc gaatgaagca aaagaaacgt gagaaggagg gtctcttgcc catctctccg
      901 gccaccccgc caggaaacga cgagaaggcc gaggaatcct cagagaagtc cagctcttcg
      961 ccctgcgttc cttccccggg gtcttctacc tcagacactc tgactacctc ccactagtaa
     1021 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1081 gacgattttt cgatcagaac tttagctag
//
