LOCUS       sequence-285855-        1178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3104
FEATURES             Location/Qualifiers
     source          1..1178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1010..1034)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1042..1083
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt tccccagccc tgctctcacg
       61 cccacgccct tctcagtcaa agacatccta aacctggaac agcagcagcg cagcctggct
      121 gccgccggag agctctctgc ccgcctggag gcgaccctgg cgccctcctc ctgcatgctg
      181 gccgccttca agccagaggc ctacgctggg cccgaggcgg ctgcgccggg cctcccagag
      241 ctgcgcgcag agctgggccg cgcgccttca ccggccaagt gtgcgtctgc ctttcccgcc
      301 gcccccgcct tctatccacg tgcctacagc gaccccgacc cagccaagga ccctagagcc
      361 gaaaagaaag agctgtgcgc gctgcagaag gcggtggagc tggagaagac agaggcggac
      421 aacgcggagc ggccccgggc gcgacggcgg aggaagccgc gcgtgctctt ctcgcaggcg
      481 caggtctatg agctggagcg gcgcttcaag cagcagcggt acctgtcggc ccccgaacgc
      541 gaccagctgg ccagcgtgct gaaactcacg tccacgcagg tcaagatctg gttccagaac
      601 cggcgctaca agtgcaagcg gcagcggcag gaccagactc tggagctggt ggggctgccc
      661 ccgccgccgc cgccgcctgc ccgcaggatc gcggtgccag tgctggtgcg cgatggcaag
      721 ccatgcctag gggactcggc gccctacgcg cctgcctacg gcgtgggcct caatccctac
      781 ggttataacg cctaccccgc ctatccgggt tacggcggcg cggcctgcag ccctggctac
      841 agctgcactg ccgcttaccc cgccgggcct tccccagcgc agccggccac tgccgccgcc
      901 aacaacaact tcgtgaactt cggcgtcggg gacttgaatg cggttcagag ccccgggatt
      961 ccgcagagca actcgggagt gtccacgctg catggtatcc gagcctggta cccaactttc
     1021 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     1081 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1141 tgtggaaagg acgatgcaac actgcacaat gaagacag
//
