LOCUS       sequence-285864-        1601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3107
FEATURES             Location/Qualifiers
     source          1..1601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1433..1457)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1465..1506
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcttca ccatggccag caacagcagc
       61 tcctgcccga cacctggggg cgggcacctc aatgggtacc cggtgcctcc ctacgccttc
      121 ttcttccccc ctatgctggg tggactctcc ccgccaggcg ctctgaccac tctccagcac
      181 cagcttccag ttagtggata tagcacacca tccccagcca ccattgagac ccagagcagc
      241 agttctgaag agatagtgcc cagccctccc tcgccacccc ctctaccccg catctacaag
      301 ccttgctttg tctgtcagga caagtcctca ggctaccact atggggtcag cgcctgtgag
      361 ggctgcaagg gcttcttccg ccgcagcatc cagaagaaca tggtgtacac gtgtcaccgg
      421 gacaagaact gcatcatcaa caaggtgacc cggaaccgct gccagtactg ccgactgcag
      481 aagtgctttg aagtgggcat gtccaaggag tctgtgagaa acgaccgaaa caagaagaag
      541 aaggaggtgc ccaagcccga gtgctctgag agctacacgc tgacgccgga ggtgggggag
      601 ctcattgaga aggtgcgcaa agcgcaccag gaaaccttcc ctgccctctg ccagctgggc
      661 aaatacacta cgaacaacag ctcagaacaa cgtgtctctc tggacattga cctctgggac
      721 aagttcagtg aactctccac caagtgcatc attaagactg tggagttcgc caagcagctg
      781 cccggcttca ccaccctcac catcgccgac cagatcaccc tcctcaaggc tgcctgcctg
      841 gacatcctga tcctgcggat ctgcacgcgg tacacgcccg agcaggacac catgaccttc
      901 tcggacgggc tgaccctgaa ccggacccag atgcacaacg ctggcttcgg ccccctcacc
      961 gacctggtct ttgccttcgc caaccagctg ctgcccctgg agatggatga tgcggagacg
     1021 gggctgctca gcgccatctg cctcatctgc ggagaccgcc aggacctgga gcagccggac
     1081 cgggtggaca tgctgcagga gccgctgctg gaggcgctaa aggtctacgt gcggaagcgg
     1141 aggcccagcc gcccccacat gttccccaag atgctaatga agattactga cctgcgaagc
     1201 atcagcgcca agggggctga gcgggtgatc acgctgaaga tggagatccc gggctccatg
     1261 ccgcctctca tccaggaaat gttggagaac tcagagggcc tggacactct gagcggacag
     1321 ccggggggtg gggggcggga cgggggtggc ctggcccccc cgccaggcag ctgtagcccc
     1381 agcctcagcc ccagctccaa cagaagcagc ccggccaccc actccccgtg agacccagct
     1441 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1501 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1561 tcttgtggaa aggacgactc ttgaccgtcc ggcaagaccc g
//
