LOCUS       sequence-285867-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3108
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(620..644)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             652..693
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggacggcgg cacactgccc
       61 aggtccgcgc cccctgcgcc ccccgtccct gtcggctgcg ctgcccggcg gagacccgcg
      121 tccccggaac tgttgcgctg cagccggcgg cggcgaccgg ccaccgcaga gaccggaggc
      181 ggcgcagcgg ccgtagcgcg gcgcaatgag cgcgagcgca accgcgtgaa gctggtgaac
      241 ttgggcttcc aggcgctgcg gcagcacgtg ccgcacggcg gcgccagcaa gaagctgagc
      301 aaggtggaga cgctgcgctc agccgtggag tacatccgcg cgctgcagcg cctgctggcc
      361 gagcacgacg ccgtgcgcaa cgcgctggcg ggagggctga ggccgcaggc cgtgcggccg
      421 tctgcgcccc gcgggccgcc agggaccacc ccggtcgccg cctcgccctc ccgcgcttct
      481 tcgtccccgg gccgcggggg cagctcggag cccggctccc cgcgttccgc ctactcgtcg
      541 gacgacagcg gctgcgaagg cgcgctgagt cctgcggagc gcgagctact cgacttctcc
      601 agctggttag ggggctactt gccaactttc ttgtacaaag tggttgatat cggtaagcct
      661 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
      721 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaagact tcggtccgta
      781 gtcttgat
//
