LOCUS       sequence-285870-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3109
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(803..827)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             835..876
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt tggactcggt gacacacagc
       61 accttcctgc ctaatgcatc cttctgcgat cccctgatgt cgtggactga tctgttcagc
      121 aatgaagagt actaccctgc ctttgagcat cagacagcct gtgactcata ctggacatca
      181 gtccaccctg aatactggac taagcgccat gtgtgggagt ggctccagtt ctgctgcgac
      241 cagtacaagt tggacaccaa ttgcatctcc ttctgcaact tcaacatcag tggcctgcag
      301 ctgtgcagca tgacacagga ggagttcgtc gaggcagctg gcctctgcgg cgagtacctg
      361 tacttcatcc tccagaacat ccgcacacaa ggttactcct tttttaatga cgctgaagaa
      421 agcaaggcca ccatcaaaga ctatgctgat tccaactgct tgaaaacaag tggcatcaaa
      481 agtcaagact gtcacagtca tagtagaaca agcctccaaa gttctcatct atgggaattt
      541 gtacgagacc tgcttctatc tcctgaagaa aactgtggca ttctggaatg ggaagatagg
      601 gaacaaggaa tttttcgggt ggttaaatcg gaagccctgg caaagatgtg gggacaaagg
      661 aagaaaaatg acagaatgac atatgaaaag ttgagcagag ccctgagata ctactataaa
      721 acaggaattt tggagcgggt tgaccgaagg ttagtgtaca aatttggaaa aaatgcacac
      781 gggtggcagg aagacaagct atgcccaact ttcttgtaca aagtggttga tatcggtaag
      841 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
      901 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagca aaggtcacag
      961 atgcctctaa t
//
