LOCUS       sequence-285873-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3110
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(596..620)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             628..669
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggaggccgc tgcagacggc
       61 ccggctgaga cccaaagccc ggtggaaaaa gacagcccgg cgaagaccca aagcccagcc
      121 caagacacct caatcatgtc gagaaataac gcagatacag gcagagttct tgccttacca
      181 gagcacaaga agaagcgcaa gggaaacttg ccagccgagt ccgttaagat cctccgcgac
      241 tggatgtata agcatcggtt taaggcctac ccttcagaag aagagaagca aatgctgtca
      301 gagaagacca atttgtcttt gttgcggatt tctaactggt ttatcaatgc tcgcagacgc
      361 attctcccgg atatgcttca acagcgtaga aacgacccca tcattggcca caaaacgggc
      421 aaagatgccc atgccaccca cctgcagagc accgaggcgt ctgtgccggc caagtcaggg
      481 ccagtggtcc agacaatgta caaagcctgc ccctgtggcc cttgccaaag ggccagatgt
      541 caagagagaa gcaaccagat ccggagtcgg cccctagcca gaagctcacc ggaattgcca
      601 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
      661 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
      721 atatcttgtg gaaaggacga taacagtttg tgtatgtggg gtca
//
