LOCUS       sequence-285876-        1988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3111
FEATURES             Location/Qualifiers
     source          1..1988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1820..1844)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1852..1893
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggcggccgt gcttagggat
       61 tcgacttcgg ttcccgtgac tgcagaagct aaacttatgg gctttacaca gggctgtgtg
      121 acctttgagg acgtggccat ttacttctcc caggaagaat gggggctcct tgatgaggct
      181 cagaggctcc tgtaccgcga tgtgatgctg gagaactttg cacttataac tgcgctggtt
      241 tgttggcatg ggatggagga tgaagagaca cctgagcaaa gtgtttctgt agaaggagta
      301 cctcaggtca ggactccaga ggccagtcca tccacccaga agattcaatc ctgtgacatg
      361 tgtgtcccat tcctgaccga cattttgcac ctgaccgatt tgcctgggca ggaactatac
      421 ttgactgggg catgtgcggt ctttcaccag gaccagaagc atcatagtgc agagaaaccc
      481 ttggaaagtg acatggacaa ggcctcattt gtgcagtgct gcctgttcca tgagtcagga
      541 atgcctttca ccagcagtga ggttgggaag gacttcctag ccccattggg cattcttcag
      601 ccgcaagcta ttgctaacta tgagaagcca aacaaaatca gcaaatgtga ggaggccttt
      661 catgttggaa taagtcatta caagtggagt caatgcagga gagagtccag ccacaaacac
      721 actttttttc accctagagt ctgcactgga aaaaggcttt atgaatctag caaatgtggg
      781 aaagcctgct gctgtgagtg ctcccttgtt cagctgcaaa gagtccaccc tggagaaagg
      841 ccttatgagt gcagtgaatg tgggaaatct tttagccaaa cctctcatct gaatgatcat
      901 cggagaatcc acactggaga aaggccttat gtgtgtggtc agtgtgggaa atcatttagc
      961 caaagagcca ccctcattaa acatcacaga gttcacactg gagaaaggcc ttacgagtgt
     1021 ggtgaatgtg ggaaatcttt tagccaaagt tccaacctta ttgaacattg cagaattcac
     1081 actggagaaa ggccttatga gtgtgatgaa tgtggaaaag cctttgggtc caaatccact
     1141 cttgttcgac accagagaac tcacacagga gaaaagccat atgagtgtgg tgaatgtggg
     1201 aaattattca gacaaagctt cagccttgtt gtacaccaga gaattcacac tacagcaagg
     1261 ccttatgagt gtggccagtg tgggaaatca tttagcctaa agtgtggcct cattcagcac
     1321 cagttaattc acagtggagc taggcccttt gagtgtgatg agtgcggaaa atcctttagc
     1381 caaagaacca ccctcaataa acaccacaaa gttcacactg cagaaaggcc ttatgtatgt
     1441 ggggaatgtg ggaaagcttt tatgttcaaa tctaaacttg ttaggcacca gagaactcac
     1501 actggagaaa ggccttttga gtgcagtgaa tgtgggaaat tttttagaca aagctatacc
     1561 ctcgttgaac accagaaaat tcacactgga ttaaggcctt acgactgtgg acagtgcggg
     1621 aaatccttta tccaaaagtc tagcctcatt caacaccaag tggttcacac aggagaaagg
     1681 ccatatgagt gtggcaaatg tgggaagtcc tttacacaac actctggcct cattctccac
     1741 cgaaaatctc acactgtgga gaggcctcgt gacagcagca aatgtggaaa accctacagc
     1801 ccaagatcta acattgttta cccaactttc ttgtacaaag tggttgatat cggtaagcct
     1861 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     1921 agtatttcga tttcttggct ttatatatct tgtggaaagg acgatgcctt gccgtagtca
     1981 aactcacc
//
