LOCUS       sequence-285879-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3112
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(821..845)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             853..894
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggagcttac ccagcctgca
       61 gaagacctca tccagaccca gcagacccct gcctcagaac ttggggaccc tgaagacccc
      121 ggagaggagg ctgcagatgg ctcagacact gtggtcctca gtctctttcc ctgcacccct
      181 gagcctgtga atcctgaacc ggatgccagt gtttcctctc cacaggcagg cagctccctg
      241 aagcactcca ccactctcac caaccggcag cgagggaacg aggtgtcagc tctgccggcc
      301 accctagact ccctgtccat ccaccagctc gcagcacagg gggagctgga ccagctgaag
      361 gagcatttgc ggaaaggtga caacctcgtc aacaagccag acgagcgcgg cttcaccccc
      421 ctcatctggg cctccgcctt tggagagatt gagaccgttc gcttcctgct ggagtggggt
      481 gccgaccccc acatcctggc aaaagagcga gagagcgccc tgtcgctggc cagcacaggc
      541 ggctacacag acattgtggg gctgctgctg gagcgtgacg tggacatcaa catctatgat
      601 tggaatggag ggacgccact gctgtacgct gtgcgcggga accacgtgaa atgcgttgag
      661 gccttgctgg cccgaggcgc tgacctcacc accgaagccg actctggcta caccccgatg
      721 gaccttgccg tggccctggg ataccggaaa gtgcaacagg tgatcgagaa ccacatcctc
      781 aagctcttcc agagcaacct ggtgcccgct gaccctgagt tgccaacttt cttgtacaaa
      841 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
      901 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      961 gacgattggc aaccgtcaga cacccttta
//
