LOCUS       sequence-285882-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3113
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(755..779)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             787..828
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggagcttac ccagcctgca
       61 gaagacctca tccagaccca gcagacccct gcctcagaac ttggggaccc tgaagacccc
      121 ggagaggagg ctgcagatgg ctcagacact gtggtcctca gtctctttcc ctgcacccct
      181 gagcctgtga atcctgaacc ggatgccagt gtttcctctc cacaggcagg cagctccctg
      241 aagcactcca ccactctcac caaccggcag cgagggaacg aggtgtcagc tctgccggcc
      301 accctagact gtgacaacct cgtcaacaag ccagacgagc gcggcttcac ccccctcatc
      361 tgggcctccg cctttggaga gattgagacc gttcgcttcc tgctggagtg gggtgccgac
      421 ccccacatcc tggcaaaaga gcgagagagc gccctgtcgc tggccagcac aggcggctac
      481 acagacattg tggggctgct gctggagcgt gacgtggaca tcaacatcta tgattggaat
      541 ggagggacgc cactgctgta cgctgtgcgc gggaaccacg tgaaatgcgt tgaggccttg
      601 ctggcccgag gcgctgacct caccaccgaa gccgactctg gctacacccc gatggacctt
      661 gccgtggccc tgggataccg gaaagtgcaa caggtgatcg agaaccacat cctcaagctc
      721 ttccagagca acctggtgcc cgctgaccct gagttgccaa ctttcttgta caaagtggtt
      781 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
      841 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
      901 gtagtagcaa aaacgctcag ttt
//
