LOCUS       sequence-285885-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3114
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(608..632)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             640..681
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaagaccaa gaaccggccc
       61 ccacggcgcc gggccccggt gcaggacaca gaggccaccc ccggggaggg gacgcccgac
      121 gggtccctgc cgaacccggg gccagagccg gccaagggtc tgcggagccg gccggcccgg
      181 gccgcagcaa gggctccggg cgagggcagg cgcaggcggc caggaccctc cgggcccggt
      241 ggccgtcgtg acagcagcat ccagcggcgg ctggagagca acgagaggga gcggcagcgg
      301 atgcacaagc taaataacgc cttccaggcc ctgcgtgaag tcatccccca cgtgcgcgcg
      361 gacaagaagc tctccaagat cgagacgctc acgctggcca agaactacat caaatcgctg
      421 acggccacca tcctgaccat gtccagcagc cgcctcccag gcctggaggg gccgggcccc
      481 aagctctacc agcactacca gcagcagcag caggtggctg ggggtgcgtt gggggccacg
      541 gaggcccagc cccagggcca cctgcagagg tactccacgc agatccacag cttccgagag
      601 ggcaccttgc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
      661 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
      721 tcttggcttt atatatcttg tggaaaggac gatgctaaca ttaccctggc ctgtcc
//
