LOCUS       sequence-285888-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3115
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(809..833)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             841..882
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc ccgccagcat gttcagcatc
       61 gacaacatcc tagccgcccg gccgcgctgc aaggactcgg tgttgccggt ggcgcacagc
      121 gcggcggctc ccgtcgtctt cccggccctg cacggggact cgctctacgg cgccagcggc
      181 ggcgcctcct cggactatgg cgccttctac ccgcgccccg tggcccccgg cggcgcgggc
      241 ctcccggccg cggtcagcgg ctcccgcctc ggctacaaca actacttcta cgggcagctg
      301 cacgtgcagg cggcgcccgt gggcccggcc tgctgcgggg ccgtgccgcc gctgggcgcc
      361 cagcagtgct cctgcgtccc gacgccccca ggctacgagg gccccggttc ggtgctggtg
      421 tccccggtac cgcaccagat gctgccctac atgaacgtgg gcacgctgtc gcgcaccgag
      481 ctgcagcttc tcaaccagct gcactgtcgg cggaagcggc ggcaccgcac catcttcact
      541 gacgagcagc tcgaagctct cgagaacctc ttccaggaga ccaagtaccc ggacgtgggc
      601 acgcgcgagc agctggcccg gaaagtgcac ctccgcgagg agaaagtgga ggtctggttt
      661 aagaaccgcc gcgccaaatg gaggcggcag aagcggtcct catcagagga gtcggagaac
      721 gcggagaagt ggaacaagac gtcgtcgtcg aaggcgtcac cggagaagag ggaagaggaa
      781 ggtaaaagcg atttggactc ggacagctgc ccaactttct tgtacaaagt ggttgatatc
      841 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
      901 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgattgtcgc
      961 cagtcatctg tatattt
//
