LOCUS       sequence-285894-        2408 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3117
FEATURES             Location/Qualifiers
     source          1..2408
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2240..2264)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2272..2313
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga ggcctgcggt gctgggctcc
       61 ccagaccgag cacccccaga agatgagggg cctgtcatgg tgaagctaga ggactctgag
      121 gaggagggtg aggctgcctt atgggaccca ggccctgaag ctgcacgcct gcgtttccgg
      181 tgcttccgct atgaggaggc cacagggccc caagaggccc tggcccagct ccgagagctg
      241 tgtcgccagt ggctgcgtcc agaggtacgc tccaaggagc agatgctgga gctgttggtg
      301 ctggagcagt tcctgggcgc actgccccct gagatccagg cccgtgtgca ggggcagcgg
      361 ccaggcagcc ccgaggaggc tgctgcccta gtagatgggc tgcgccggga gccgggcgga
      421 ccccggagat gggtcacagt ccaggtgcag ggccaggagg tcctatcaga gaagatggag
      481 ccctccagtt tccagcccct acctgaaact gagcctccaa ctccagagcc tgggcccaag
      541 acacctccta ggactatgca ggaatcacca ctgggcctgc aggtgaaaga ggagtcagag
      601 gttacagagg actcagattt cctggagtct gggcctctag ctgccaccca ggagtctgta
      661 cccaccctcc tgcctgagga ggcccagaga tgtgggaccg tgctggacca gatctttccc
      721 cacagcaaga ctgggcctga gggtccctca tggagggagc accccagggc cctgtggcat
      781 gaggaagctg ggggcatctt ctccccaggg ttcgcgctgc agctaggcag catctccgca
      841 ggtccaggta gtgtaagccc tcacctccac gtcccctggg acctcggcat ggctggcctt
      901 tctggccaga tccaatcacc ctcccgcgaa ggtggctttg cgcatgcgct tctgctcccc
      961 agcgatctga ggagtgaaca ggaccccacg gacgaggatc cctgccgggg tgtgggccct
     1021 gctctgatca ccacccgctg gcgctccccc aggggccgga gccggggccg ccccagcact
     1081 gggggcgggg tggttagggg cggccgttgc gatgtatgtg gcaaggtgtt cagccaacgc
     1141 agcaacctgc tgaggcacca gaagatccac acgggtgagc gaccattcgt gtgcagcgag
     1201 tgcggccgca gcttcagccg cagctcgcac ctgctgcgcc accagcttac gcacaccgag
     1261 gagcggccgt tcgtgtgcgg cgactgtggc cagggcttcg tgcgcagcgc gcgcctggaa
     1321 gagcatcgga gagtgcacac gggcgaacag cctttccgtt gcgctgagtg cggccagagc
     1381 ttccggcagc gctccaatct gctgcagcac cagcgcatcc acggcgatcc cccgggccct
     1441 ggcgctaagc ccccggcccc tcctggtgcg cccgagcctc ccggcccctt tccgtgcagc
     1501 gagtgccgcg agagcttcgc gcggcgcgcc gtgctgctgg agcaccaggc ggtacacacg
     1561 ggcgacaagt cctttggctg cgtcgagtgc ggcgagcgct tcggccgccg ctcagtgctg
     1621 ctgcagcacc ggcgcgtgca cagtggcgag cggcccttcg cctgtgccga gtgcggccag
     1681 agcttccggc agcgctccaa cctgacgcag caccggcgca tccacaccgg ggagcggccc
     1741 ttcgcctgcg ccgagtgtgg caaggccttc cgccagcggc ctacgctcac gcagcatctc
     1801 cgcgtacaca cgggcgagaa accctttgcc tgccccgagt gtggccagcg cttcagccag
     1861 cgcctcaagc tcacgcgtca tcagaggaca cacaccggcg aaaagcccta ccactgcggt
     1921 gagtgcggcc tgggcttcac gcaggtctcg cggctcaccg agcaccagcg catccacacg
     1981 ggcgaacggc ccttcgcctg ccccgagtgc ggccagagct ttcggcagca cgccaacctc
     2041 acccagcacc ggcgcatcca cacgggtgaa cggccctacg catgccctga gtgtggcaag
     2101 gccttccgcc agcggcccac gctcacgcag catctgcgca cccaccgacg agagaagccc
     2161 ttcgcctgcc aggactgtgg ccgccgcttc caccagagca ccaagctcat tcagcaccag
     2221 cgcgtccaca gcgccgagta cccaactttc ttgtacaaag tggttgatat cggtaagcct
     2281 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     2341 agtatttcga tttcttggct ttatatatct tgtggaaagg acgagttcga aactaatagt
     2401 cgcccttt
//
