LOCUS       sequence-285897-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3118
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(629..653)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             661..702
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc tggtgctgcc atccccctgc
       61 cctcagcctc tggcattttc ctccgttgag accatggagg gccctccccg tcggacttgc
      121 cgctccccag aacctggacc ttcctcctcc atcggatctc cccaggcttc atctcctcca
      181 aggcccaacc actacctgct tattgacact cagggtgtcc cctacacagt gctggtggac
      241 gaggagtcac agagggagcc aggggccagt ggggctccag gccagaaaaa gtgctacagc
      301 tgccccgtgt gctcaagggt cttcgagtac atgtcctacc ttcagcgaca cagcatcacc
      361 cactcggagg taaagccctt cgagtgtgac atctgtggga aggcattcaa gcgcgccagc
      421 cacttggcac ggcaccattc cattcacctg gcgggtggtg ggcggcccca cggctgcccg
      481 ctctgccctc gccgcttccg ggatgcgggt gagctggccc agcacagccg ggtgcactct
      541 ggggagcgcc cgtttcagtg tccacactgc cctcgccgct ttatggagca gaacacactg
      601 cagaaacaca cgcggtggaa gcatccatgc ccaactttct tgtacaaagt ggttgatatc
      661 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
      721 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaattatgt
      781 ttagaactaa tatctag
//
