LOCUS       sequence-285900-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3119
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(545..569)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             577..618
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cagctgagtc attgcctttc
       61 tcctttggga cactgtccag ctgggagctg gaagcctggt atgaggacct gcaagaggtc
      121 ctgtcttcag atgaaaatgg gggtacctat gtttcacctc ctggaaatga agaggaagaa
      181 tcaaaaatct tcaccactct tgaccctgct tctctggctt ggctgactga ggaggagcca
      241 gaaccagcag aggtcacaag cacctcccag agccctcact ctccagattc cagtcagagc
      301 tccctggctc aggaggaaga ggaggaagac caagggagaa ccaggaaacg gaaacagagt
      361 ggtcattccc cagcccgggc tggaaagcag cgcatgaagg agaaagaaca ggagaatgaa
      421 aggaaagtgg cacagctagc tgaagagaat gaacggctca agcaggaaat cgagcgcctg
      481 accagggaag tagaggcgac tcgccgagct ctgattgacc gaatggtgaa tctgcaccaa
      541 gcatgcccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
      601 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
      661 tggctttata tatcttgtgg aaaggacgag actcgagtat aagtgcgata ctt
//
