LOCUS       sequence-285903-        2771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3120
FEATURES             Location/Qualifiers
     source          1..2771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2603..2627)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2635..2676
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcgcaggc gatctttgag
       61 gccctggagg gaatggacaa tcagaccgtt ctggctgtcc agtcattatt ggatggccaa
      121 ggagcagtcc ctgatccgac aggccagagt gtcaatgcgc cccctgctat ccagccattg
      181 gatgacgagg atgtatttct ctgcgggaag tgtaagaagc aattcaactc gctgccagcg
      241 tttatgaccc acaagcggga acagtgccag gggaatgccc ccgccctggc cacagtctca
      301 ctggccacca acagcatcta cccaccttcg gcagcaccca cagcggtcca gcaggcccca
      361 actcctgcca atcgccagat ctccacatac atcacagtgc ccccgtcccc actgatccag
      421 accctggtgc aggggaacat cttggtgagc gatgatgtgc tcatgtctgc catgtcagcc
      481 ttcacatccc tggaccagcc catgccccag ggccccccac ctgtgcagag cagcctgaac
      541 atgcattccg tgcccagcta cctcacccag cctccacctc ctcctccacc tcctccacca
      601 ctgcccccac cgccaccacc tcagcctcca ccacctccac cccagagcct gggcccccct
      661 gggcgtccca accctggtgg gaacggtgtg gtggaggtgt acagtgctgc tgcgcccctg
      721 gctgggagtg gaacggtgga gatccaggca ctggggatgc agccctaccc acccctagag
      781 gtgccaaacc agtgtgtgga gcctccagta tatcccaccc ccacagtgta cagccctggc
      841 aaacagggat tcaaacccaa aggaccaaac cccgccgccc ccatgaccag cgccaccggg
      901 ggcacggtgg ccacctttga ctctccagca acgctgaaga cccgacgagc taaaggtgcc
      961 aggggactcc cggaagctgc agggaagcca aaggctcaga aactcaagtg ctcatactgt
     1021 gacaagtcat tcaccaaaaa ctttgacctg cagcagcaca tccgaagcca caccggtgag
     1081 aagcccttcc agtgcattgc atgtggccgt gcctttgccc agaagtctaa tgttaagaaa
     1141 cacatgcaga cccacaaggt gtggcctcca ggacacagtg gtggcaccgt gtctcgaaac
     1201 tctgtgaccg tacaggtcat ggccctgaac cccagcaggc aggaggacga ggaaagcaca
     1261 gggttgggcc agcccctgcc gggtgcgcca cagccccagg ccttgtccac agctggtgag
     1321 gaagaggggg acaagccgga gtccaagcag gtggtcctca tcgacagctc ctacctgtgc
     1381 caattctgcc ccagcaaatt cagcacctac ttccagctca agtctcacat gacccagcat
     1441 aagaatgagc aggtgtacaa gtgtgtggtc aaaagctgtg cccagacgtt cccaaagctc
     1501 gacacatttc tggagcacat caagagccac caggaggagc tgagctaccg ctgccacctc
     1561 tgcggcaagg acttcccctc gctgtacgac ctgggcgtgc accagtactc ccacagcctc
     1621 ctgccacagc acagccccaa gaaggacaat gccgtctaca agtgtgtcaa atgtgtcaac
     1681 aaatactcca cccctgaggc cctggagcac cacctgcaga ccgccactca caacttcccc
     1741 tgcccacact gccagaaggt gtttccttgt gaacgctacc tgcggcgtca tctgcccacc
     1801 cacggcagcg ggggcaggtt caagtgccaa gtgtgcaaga agttcttccg gcgggagcat
     1861 tatctcaaac tgcatgctca catccactcg ggtgagaagc cctacaaatg ctcagtgtgc
     1921 gagtctgcgt tcaaccgcaa ggacaaactg aagagacaca tgttgatcca cgagcccttc
     1981 aagaaataca aatgcccttt ctcgacgcac acaggctgca gtaaggagtt caaccggccg
     2041 gacaagctga aggcccacat cctctcccac tctggcatga agctccacaa atgcgccctg
     2101 tgcagcaagt ccttcagccg ccgtgcccac ctcgccgagc atcagcgcgc ccacacgggc
     2161 aactacaagt tccgctgtgc tggctgcgcc aagggctttt cccgccacaa atacctcaaa
     2221 gatcaccgct gtcgtctcgg cccccaaaag gacaaggacc tgcaaacccg gcggcccccc
     2281 cagaggaggg cagccccccg cagttgcggc agtggtgggc gcaaggtgct gacccccttg
     2341 cctgacccgc tggggctgga ggagctgaag gacacagggg ctgggctggt gcccgaggct
     2401 gtccccggca agccgccctt cgcagagccg gacgcggtgc tgtccatcgt tgtgggtggt
     2461 gcggtgggcg cggaaactga gctggtggta cctggacacg ctgaggggct gggctccaac
     2521 ctggctctgg cggagctgca ggccggggcc gagggcccat gtgccatgct cgctgtgccc
     2581 gtctacatcc aggcctccga gttgccaact ttcttgtaca aagtggttga tatcggtaag
     2641 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     2701 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgattc caggataggg
     2761 gcgcacgctc c
//
