LOCUS       sequence-285909-        2495 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3122
FEATURES             Location/Qualifiers
     source          1..2495
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2327..2351)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2359..2400
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg atcatttgaa cgaggcaact
       61 caggggaaag aacattcaga aatgtctaac aatgtgagtg atccgaaggg tccaccagcc
      121 aagattgccc gcctggagca gaacgggagc ccgctaggaa gaggaaggct tgggagtaca
      181 ggtgcaaaaa tgcagggagt gcctttaaaa cactcgggcc atctgatgaa aaccaacctt
      241 aggaaaggaa ccatgctgcc agttttctgt gtggtggaac attatgaaaa cgccattgaa
      301 tatgattgca aggaggagca tgcagaattt gtgctggtga gaaaggatat gcttttcaac
      361 cagctgatcg aaatggcatt gctgtctcta ggttattcac atagctctgc tgcccaggcc
      421 aaagggctaa tccaggttgg aaagtggaat ccagttccac tgtcttacgt gacagatgcc
      481 cctgatgcta cagtagcaga tatgcttcaa gatgtgtatc atgtggtcac attgaaaatt
      541 cagttacaca gttgccccaa actagaagac ttgcctcccg aacaatggtc gcacaccaca
      601 gtgaggaatg ctctgaagga cttactgaaa gatatgaatc agagttcatt ggccaaggag
      661 tgcccccttt cacagagtat gatttcttcc attgtgaaca gtacttacta tgcaaatgtc
      721 tcagcagcaa aatgtcaaga atttggaagg tggtacaaac atttcaagaa gacaaaagat
      781 atgatggttg aaatggatag tctttctgag ctatcccagc aaggcgccaa tcatgtcaat
      841 tttggccagc aaccagttcc agggaacaca gccgagcagc ctccatcccc tgcgcagctc
      901 tcccatggca gccagccctc tgtccggaca cctcttccaa acctgcaccc tgggctcgta
      961 tcaacaccta tcagtcctca attggtcaac cagcagctgg tgatggctca gctgctgaac
     1021 cagcagtatg cagtgaatag acttttagcc cagcagtcct taaaccaaca atacttgaac
     1081 caccctcccc ctgtcagtag atctatgaat aagcctttgg agcaacaggt ttcgaccaac
     1141 acagaggtgt cttccgaaat ctaccagtgg gtacgcgatg aactgaaacg agcaggaatc
     1201 tcccaggcgg tatttgcacg tgtggctttt aacagaactc agggcttgct ttcagaaatc
     1261 ctccgaaagg aagaggaccc caagactgca tcccagtctt tgctggtaaa ccttcgggct
     1321 atgcagaatt tcttgcagtt accggaagct gaaagagacc gaatatacca ggacgaaagg
     1381 gaaaggagct tgaatgctgc ctcggccatg ggtcctgccc ccctcatcag cacaccaccc
     1441 agccgtcctc cccaggtgaa aacagctact attgccactg aaaggaatgg gaaaccagag
     1501 aacaatacca tgaacattaa tgcttccatt tatgatgaga ttcagcagga aatgaagcgt
     1561 gctaaagtgt ctcaagcact gtttgcaaag gttgcagcaa ccaaaagcca gggatggttg
     1621 tgcgagctgt tacgctggaa agaagatcct tctccagaaa acagaaccct gtgggagaac
     1681 ctctccatga tccgaaggtt cctcagtctt cctcagccag aacgtgatgc catttatgaa
     1741 caggagagca acgcggtgca tcaccatggc gacaggccgc cccacattat ccatgttcca
     1801 gcagagcaga ttcagcaaca gcagcagcaa cagcaacagc agcagcagca gcagcaggca
     1861 ccgccgcctc cacagccaca gcagcagcca cagacaggcc ctcggctccc cccacggcaa
     1921 cccacggtgg cctctccagc agagtcagat gaggaaaacc gacagaagac ccggccacga
     1981 acaaaaattt cagtggaagc cttgggaatc ctccagagtt tcatacaaga cgtgggcctg
     2041 taccctgacg aagaggccat ccagactctg tctgcccagc tcgaccttcc caagtacacc
     2101 atcatcaagt tctttcagaa ccagcggtac tatctcaagc accacggcaa actgaaggac
     2161 aattccggtt tagaggtcga tgtggcagaa tataaagaag aggagctgct gaaggatttg
     2221 gaagagagtg tccaagataa aaatactaac accctttttt cagtgaaact agaagaagag
     2281 ctgtcagtgg aaggaaacac agacattaat actgatttga aagactgccc aactttcttg
     2341 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     2401 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2461 ggaaaggacg aaaaccatct ccgacatgaa tgtta
//
