LOCUS       sequence-285912-        2450 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3123
FEATURES             Location/Qualifiers
     source          1..2450
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2282..2306)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2314..2355
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ctcagtggaa tcaagtccaa
       61 cagttagaaa tcaagttttt ggagcaggtg gatcaattct atgatgacaa ctttcccatg
      121 gaaattcggc atctgttggc ccaatggatt gaaaatcaag actgggaggc agcttctaac
      181 aatgaaacca tggcaacgat tcttcttcaa aacttgttaa tacaactgga tgaacagtta
      241 ggtcgtgttt ccaaagagaa aaacctactc ttgatacaca atctaaaaag aattaggaag
      301 gtccttcagg gaaaatttca tggaaatcca atgcatgtag ctgtggttat ttcaaactgt
      361 ttaagggaag agaggagaat attggctgca gccaacatgc ctgtccaggg gcctctagag
      421 aaatccttac aaagttcttc agtttcagaa agacagagga atgtggagca caaagtggct
      481 gccattaaaa acagtgtgca gatgacagaa caagatacca aatacttaga agatctgcaa
      541 gacgaatttg actacaggta taaaacaatt cagacaatgg atcagagtga caagaatagt
      601 gccatggtga atcaggaagt tttgacactg caggaaatgc ttaacagcct cgatttcaag
      661 agaaaggagg ctctcagtaa aatgacccaa atcatccatg agacagacct gttaatgaac
      721 accatgctca tagaagagct gcaagactgg aagcggcggc agcaaatcgc ctgcatcggg
      781 ggtccactcc acaatgggct cgaccagctt cagaactgct ttacactatt ggcagaaagt
      841 cttttccaac tgagaaggca attggagaaa ctagaggagc aatctaccaa aatgacatat
      901 gaaggtgatc ccattccaat gcaaagaact cacatgctag aaagagtcac cttcttgatc
      961 tacaaccttt tcaagaactc atttgtggtt gagcgacagc catgtatgcc aacccaccct
     1021 cagaggccgt tggtacttaa aaccctaatt cagttcactg taaaactaag gctactaata
     1081 aaattgccag aactaaacta tcaggtaaag gttaaggcat caattgacaa gaatgtttca
     1141 actctaagca accgaagatt tgtactttgt ggaactaatg tcaaagccat gtctattgaa
     1201 gaatcttcca atgggagtct ctcagtagaa tttcgacatt tgcaaccaaa ggaaatgaag
     1261 tccagtgctg gaggtaaagg aaatgagggc tgtcacatgg tgactgaaga acttcattcc
     1321 ataacgtttg aaacacagat ctgcctctat ggcctgacca tagatttgga gaccagctca
     1381 ttgcctgtgg tgatgatttc caatgtcagt cagttaccta atgcttgggc atccatcatt
     1441 tggtacaacg tgtcaaccaa cgattcccag aacttggttt tctttaataa tcctccacct
     1501 gccacattga gtcaactact ggaggtgatg agctggcagt tttcatcgta cgttggtcgt
     1561 ggtcttaact cagatcaact ccatatgctg gcagagaagc ttacagtcca atctagctac
     1621 agtgatggtc acctcacctg ggccaagttc tgcaaggaac atttacctgg taaatcattt
     1681 accttttgga catggcttga agcaatattg gatctaatta agaaacacat tcttcccctt
     1741 tggattgatg ggtatgtcat gggctttgtt agcaaagaga aggaacggct gttgctaaag
     1801 gataaaatgc ctggcacctt tttattaaga ttcagtgaaa gccatctcgg aggaataact
     1861 ttcacctggg tggaccattc tgaaagtggg gaagtgagat tccactctgt agaaccctac
     1921 aataaaggcc ggttgtctgc tctgccattc gctgacatcc tgcgagacta caaagttatt
     1981 atggctgaaa acattcctga aaaccctctg aagtacctat atcctgacat tcccaaagac
     2041 aaagccttcg gtaaacacta cagctctcag ccttgcgaag tttcaagacc aacagaaagg
     2101 ggtgacaaag gttatgttcc ttctgttttt atccccatct caacaatccg aagtgattca
     2161 acagagccac attctccatc agaccttctt cccatgtctc caagtgtgta tgcggtgttg
     2221 agagaaaacc tgagtcccac aacaattgaa actgcaatga agtctcctta ttctgctgaa
     2281 tgcccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     2341 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     2401 ctttatatat cttgtggaaa ggacgaagat cctcagttgc ccggatggtt
//
