LOCUS       sequence-285915-        1481 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3124
FEATURES             Location/Qualifiers
     source          1..1481
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1313..1337)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1345..1386
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cgtccatcct gcctttcact
       61 cccccgatcg tgaagcgcct gctgggctgg aagaagggcg agcagaacgg gcaggaggag
      121 aaatggtgcg agaaggcggt caagagcctg gtcaagaaac tcaagaagac ggggcagctg
      181 gacgagctgg agaaggccat caccacgcag aacgtcaaca ccaagtgcat caccatcccc
      241 aggtccctgg atggccggtt gcaggtgtcc catcggaagg ggctccctca tgtcatctac
      301 tgccgcctgt ggcgatggcc agacctgcac agccaccacg agctgcgggc catggagctg
      361 tgtgagttcg ccttcaatat gaagaaggac gaggtctgcg tgaatcccta ccactaccag
      421 agagtagaga caccagttct acctcctgtg ttggtgccac gccacacaga gatcccggcc
      481 gagttccccc cactggacga ctacagccat tccatccccg aaaacactaa cttccccgca
      541 ggcatcgagc cccagagcaa tattccagag accccacccc ctggctacct gagtgaagat
      601 ggagaaacca gtgaccacca gatgaaccac agcatggacg caggttctcc aaacctatcc
      661 ccgaatccga tgtccccagc acataataac ttggacctgc agccagttac ctactgcgag
      721 ccggccttct ggtgctccat ctcctactac gagctgaacc agcgcgtcgg ggagacattc
      781 cacgcctcgc agccatccat gactgtggat ggcttcaccg acccctccaa ttcggagcgc
      841 ttctgcctag ggctgctctc caatgtcaac aggaatgcag cagtggagct gacacggaga
      901 cacatcggaa gaggcgtgcg gctctactac atcggagggg aggtcttcgc agagtgcctc
      961 agtgacagcg ctatttttgt ccagtctccc aactgtaacc agcgctatgg ctggcacccg
     1021 gccaccgtct gcaagatccc accaggatgc aacctgaaga tcttcaacaa ccaggagttc
     1081 gctgccctcc tggcccagtc ggtcaaccag ggctttgagg ctgtctacca gttgacccga
     1141 atgtgcacca tccgcatgag cttcgtcaaa ggctggggag cggagtacag gagacagact
     1201 gtgaccagta ccccctgctg gattgagctg cacctgaatg ggcctttgca gtggcttgac
     1261 aaggtcctca cccagatggg ctccccaagc atccgctgtt ccagtgtgtc ttacccaact
     1321 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1381 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1441 tcttgtggaa aggacgacaa acttggttgg catgcaaaga t
//
